TY - JOUR
T1 - Development of an AND logic-gate-type fluorescent probe for ratiometric imaging of autolysosome in cell autophagy
AU - Takashima, Ippei
AU - Kawagoe, Ryosuke
AU - Hamachi, Itaru
AU - Ojida, Akio
N1 - Publisher Copyright:
© 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/1/26
Y1 - 2015/1/26
N2 - The concomitant detection of two biological events facilitates the highly selective and sensitive analysis of specific biological functions. In this article, we report an AND logic-gate-type fluorescent probe that can concurrently sense two biological events in living cells: H2O2 accumulation and acidification. The probe exhibits a unique fluorescence sensing mechanism, in which a xanthene fluorophore is oxidatively transformed to a xanthone derivative by H2O2 , thereby resulting in a clear dual-emission change. This transformation is significantly accelerated under weak acidic conditions, which enables the selective and sensitive detection of H2O2 production in an acidic cellular compartment. This unique sensing property was successfully applied to the ratiometric fluorescence imaging of autolysosome formation in selective mitochondrial autophagy (mitophagy), which highlights the utility of this novel probe in autophagy research.
AB - The concomitant detection of two biological events facilitates the highly selective and sensitive analysis of specific biological functions. In this article, we report an AND logic-gate-type fluorescent probe that can concurrently sense two biological events in living cells: H2O2 accumulation and acidification. The probe exhibits a unique fluorescence sensing mechanism, in which a xanthene fluorophore is oxidatively transformed to a xanthone derivative by H2O2 , thereby resulting in a clear dual-emission change. This transformation is significantly accelerated under weak acidic conditions, which enables the selective and sensitive detection of H2O2 production in an acidic cellular compartment. This unique sensing property was successfully applied to the ratiometric fluorescence imaging of autolysosome formation in selective mitochondrial autophagy (mitophagy), which highlights the utility of this novel probe in autophagy research.
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U2 - 10.1002/chem.201405686
DO - 10.1002/chem.201405686
M3 - Article
C2 - 25470007
AN - SCOPUS:84921412370
VL - 21
SP - 2038
EP - 2044
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
SN - 0947-6539
IS - 5
ER -