Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.

Michihiko Nakano, Keita Murofushi, Satoshi Watabe, Toshiya Ohta

Research output: Contribution to journalArticlepeer-review

Abstract

It was developed that an anti-norovirus single-chain Fv (scFv) antibody for immunochromathographic test kit by using the phage displayed technique and biopanning. To obtain the scFv having wide reactivity for several norovirus genotypes, a mixture of recombinant norovirus capsid proteins, including 11 norovirus genotypes, was used for biopanning. Then, one anti-norovirus scFv antibody that recognized both of norovirus genogroups GI and GII was identified. An immunochromatographic test strip was constructed by using the scFv and demonstrated it to detect norovirus in stool samples. The immunochromatographic strip showed similar sensitivity to a commercially available one on which several monoclonal antibodies are included.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalUnknown Journal
Volume23
Issue number2
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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