Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin

Seiichi Sakamoto, Waraporn Putalun, Ryota Tsuchihashi, Satoshi Morimoto, Junei Kinjo, Hiroyuki Tanaka

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Plumbagin (PL; 5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural compound mainly isolated from Plumbago zeylanica. This plant is distributed in Southeast Asia, and well known as Ayurvedic medicine in India for its medicinal properties. PL has been shown to have various pharmacological activities. We have successfully prepared monoclonal antibodies against PL, and developed an enzyme-linked immunosorbent assay (ELISA) system for determination of PL. 3-(5-Hydroxy-2-methyl-1,4-naphthoquinone-3-yl) propanoic acid was synthesized and purified to prepare PL-bovine serum albumin conjugate (PL-BSA), which was used as an immunogen. PL-BSA conjugate was administered into BALB/c male mice for production of monoclonal antibodies against PL. The monoclonal antibody against PL which is secreted from established hybridoma cell line 3A3 (MAb 3A3) has been proven to have highly-specific to PL resulting from cross-reactivities test. The range for calibration of PL by ELISA was 0.2-25 μg mL-1. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of PL in plant.

Original languageEnglish
Pages (from-to)100-105
Number of pages6
JournalAnalytica Chimica Acta
Volume607
Issue number1
DOIs
Publication statusPublished - Jan 21 2008

Fingerprint

Immunosorbents
antibody
Assays
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
assay
enzyme
Bovine Serum Albumin
serum
Enzymes
Plumbaginaceae
Ayurvedic Medicine
Southeastern Asia
Hybridomas
medicine
Calibration
Medicine
India
analytical method
Cells

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy

Cite this

Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin. / Sakamoto, Seiichi; Putalun, Waraporn; Tsuchihashi, Ryota; Morimoto, Satoshi; Kinjo, Junei; Tanaka, Hiroyuki.

In: Analytica Chimica Acta, Vol. 607, No. 1, 21.01.2008, p. 100-105.

Research output: Contribution to journalArticle

Sakamoto, Seiichi ; Putalun, Waraporn ; Tsuchihashi, Ryota ; Morimoto, Satoshi ; Kinjo, Junei ; Tanaka, Hiroyuki. / Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin. In: Analytica Chimica Acta. 2008 ; Vol. 607, No. 1. pp. 100-105.
@article{61d6b32f47c840abbafb02cf6ad9095c,
title = "Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin",
abstract = "Plumbagin (PL; 5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural compound mainly isolated from Plumbago zeylanica. This plant is distributed in Southeast Asia, and well known as Ayurvedic medicine in India for its medicinal properties. PL has been shown to have various pharmacological activities. We have successfully prepared monoclonal antibodies against PL, and developed an enzyme-linked immunosorbent assay (ELISA) system for determination of PL. 3-(5-Hydroxy-2-methyl-1,4-naphthoquinone-3-yl) propanoic acid was synthesized and purified to prepare PL-bovine serum albumin conjugate (PL-BSA), which was used as an immunogen. PL-BSA conjugate was administered into BALB/c male mice for production of monoclonal antibodies against PL. The monoclonal antibody against PL which is secreted from established hybridoma cell line 3A3 (MAb 3A3) has been proven to have highly-specific to PL resulting from cross-reactivities test. The range for calibration of PL by ELISA was 0.2-25 μg mL-1. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of PL in plant.",
author = "Seiichi Sakamoto and Waraporn Putalun and Ryota Tsuchihashi and Satoshi Morimoto and Junei Kinjo and Hiroyuki Tanaka",
year = "2008",
month = "1",
day = "21",
doi = "10.1016/j.aca.2007.11.021",
language = "English",
volume = "607",
pages = "100--105",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin

AU - Sakamoto, Seiichi

AU - Putalun, Waraporn

AU - Tsuchihashi, Ryota

AU - Morimoto, Satoshi

AU - Kinjo, Junei

AU - Tanaka, Hiroyuki

PY - 2008/1/21

Y1 - 2008/1/21

N2 - Plumbagin (PL; 5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural compound mainly isolated from Plumbago zeylanica. This plant is distributed in Southeast Asia, and well known as Ayurvedic medicine in India for its medicinal properties. PL has been shown to have various pharmacological activities. We have successfully prepared monoclonal antibodies against PL, and developed an enzyme-linked immunosorbent assay (ELISA) system for determination of PL. 3-(5-Hydroxy-2-methyl-1,4-naphthoquinone-3-yl) propanoic acid was synthesized and purified to prepare PL-bovine serum albumin conjugate (PL-BSA), which was used as an immunogen. PL-BSA conjugate was administered into BALB/c male mice for production of monoclonal antibodies against PL. The monoclonal antibody against PL which is secreted from established hybridoma cell line 3A3 (MAb 3A3) has been proven to have highly-specific to PL resulting from cross-reactivities test. The range for calibration of PL by ELISA was 0.2-25 μg mL-1. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of PL in plant.

AB - Plumbagin (PL; 5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural compound mainly isolated from Plumbago zeylanica. This plant is distributed in Southeast Asia, and well known as Ayurvedic medicine in India for its medicinal properties. PL has been shown to have various pharmacological activities. We have successfully prepared monoclonal antibodies against PL, and developed an enzyme-linked immunosorbent assay (ELISA) system for determination of PL. 3-(5-Hydroxy-2-methyl-1,4-naphthoquinone-3-yl) propanoic acid was synthesized and purified to prepare PL-bovine serum albumin conjugate (PL-BSA), which was used as an immunogen. PL-BSA conjugate was administered into BALB/c male mice for production of monoclonal antibodies against PL. The monoclonal antibody against PL which is secreted from established hybridoma cell line 3A3 (MAb 3A3) has been proven to have highly-specific to PL resulting from cross-reactivities test. The range for calibration of PL by ELISA was 0.2-25 μg mL-1. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of PL in plant.

UR - http://www.scopus.com/inward/record.url?scp=37249018589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37249018589&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2007.11.021

DO - 10.1016/j.aca.2007.11.021

M3 - Article

C2 - 18155415

AN - SCOPUS:37249018589

VL - 607

SP - 100

EP - 105

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1

ER -