Development of an enzyme linked immunosorbent assay for direct determination of anticancer drug vitamin K3 in serum

Seiichi Sakamoto, Junichi Sakoda, Osamu Morinaga, Putalun Waraporn, Ryota Tsuchihashi, Satoshi Morimoto, Junei Kinjo, Hiroyuki Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Synthetic vitamin K3 (VK3, 2-methyl-1,4-naphthoquinone, or menadione) has been shown to have anticancer activity in various human cancer cells. We developed an enzyme-linked immunosorbant assay (ELISA) for determination of VK3 in fetal calf serum (FCS) without any pretreatment. The monoclonal antibody against VK3, which was secreted from an established hybridoma cell line (3A3) has been proven to have highly specific binding to VK3 in cross-reactivity analysis. The full measuring range of the assay extends from 0.39 to 50 μg/ml of VK3. Based on validation analysis, this immunological analysis is a precise, accurate, and sensitive method for determination of VK3. This simple immunological method would deserve to be applicable clinically.

Original languageEnglish
Pages (from-to)508-511
Number of pages4
JournalJournal of Health Science
Volume54
Issue number4
DOIs
Publication statusPublished - Aug 2008

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Vitamin K 3
Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
Enzymes
Serum
Cells
Pharmaceutical Preparations
Hybridomas
Monoclonal Antibodies
Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Development of an enzyme linked immunosorbent assay for direct determination of anticancer drug vitamin K3 in serum. / Sakamoto, Seiichi; Sakoda, Junichi; Morinaga, Osamu; Waraporn, Putalun; Tsuchihashi, Ryota; Morimoto, Satoshi; Kinjo, Junei; Tanaka, Hiroyuki.

In: Journal of Health Science, Vol. 54, No. 4, 08.2008, p. 508-511.

Research output: Contribution to journalArticle

Sakamoto, Seiichi ; Sakoda, Junichi ; Morinaga, Osamu ; Waraporn, Putalun ; Tsuchihashi, Ryota ; Morimoto, Satoshi ; Kinjo, Junei ; Tanaka, Hiroyuki. / Development of an enzyme linked immunosorbent assay for direct determination of anticancer drug vitamin K3 in serum. In: Journal of Health Science. 2008 ; Vol. 54, No. 4. pp. 508-511.
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