Development of an Enzyme-Linked Immunosorbent Assay for Specific Detection of Mulberroside A in Mulberry (Morus alba L.) Using Anti-mulberroside A Polyclonal Antibody

Jukrapun Komaikul, Tharita Kitisripanya, Hiroyuki Tanaka, Boonchoo Sritularak, Waraporn Putalun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against mulberroside A (MuA), a major active component found in the root bark of mulberry (Morus alba L.). MuA was conjugated with the carrier protein bovine serum albumin for immunization to rabbits. The results showed that the antibodies were specific only for MuA and have very low specificity for its aglycone, oxyresveratrol. The ELISA assay was suitable for quantitating MuA in the range of 0.17-15.62 μg/mL with a relative standard deviation (RSD) of less than 5 % for both intra- and inter-assay precision levels. The recovery rates of MuA in the samples were in the range of 97.6-101.4 % with a RSD of less than 5 %. The developed ELISA exhibited a good correlation value (R 2 = 0.994) with the standard high-performance liquid chromatography method in the crude extracts of plant samples. These results suggest that the developed ELISA method using PAb against MuA can be applied to determine MuA content with high specificity, rapidity, and simplicity in mulberry samples. The developed ELISA method described could prove to be useful as an analytical tool for quality control of mulberry and their products.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalFood Analytical Methods
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

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Morus
Immunosorbents
Morus alba
mulberries
polyclonal antibodies
Antibodies
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
quality control
assays
transport proteins
bovine serum albumin
plant extracts
sampling
Immunization
immunization
bark
high performance liquid chromatography

All Science Journal Classification (ASJC) codes

  • Food Science
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Development of an Enzyme-Linked Immunosorbent Assay for Specific Detection of Mulberroside A in Mulberry (Morus alba L.) Using Anti-mulberroside A Polyclonal Antibody. / Komaikul, Jukrapun; Kitisripanya, Tharita; Tanaka, Hiroyuki; Sritularak, Boonchoo; Putalun, Waraporn.

In: Food Analytical Methods, Vol. 7, No. 1, 01.01.2014, p. 58-63.

Research output: Contribution to journalArticle

Komaikul, Jukrapun ; Kitisripanya, Tharita ; Tanaka, Hiroyuki ; Sritularak, Boonchoo ; Putalun, Waraporn. / Development of an Enzyme-Linked Immunosorbent Assay for Specific Detection of Mulberroside A in Mulberry (Morus alba L.) Using Anti-mulberroside A Polyclonal Antibody. In: Food Analytical Methods. 2014 ; Vol. 7, No. 1. pp. 58-63.
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