TY - JOUR
T1 - Development of an Enzyme-Linked Immunosorbent Assay for Specific Detection of Mulberroside A in Mulberry (Morus alba L.) Using Anti-mulberroside A Polyclonal Antibody
AU - Komaikul, Jukrapun
AU - Kitisripanya, Tharita
AU - Tanaka, Hiroyuki
AU - Sritularak, Boonchoo
AU - Putalun, Waraporn
N1 - Funding Information:
Acknowledgments This work was supported in part by the Kanae Foundation for the Promotion of Medical Science, Japan; the Faculty of Pharmaceutical Science, Khon Kaen University and the Graduate School, Khon Kaen University, Thailand.
PY - 2014/1
Y1 - 2014/1
N2 - An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against mulberroside A (MuA), a major active component found in the root bark of mulberry (Morus alba L.). MuA was conjugated with the carrier protein bovine serum albumin for immunization to rabbits. The results showed that the antibodies were specific only for MuA and have very low specificity for its aglycone, oxyresveratrol. The ELISA assay was suitable for quantitating MuA in the range of 0.17-15.62 μg/mL with a relative standard deviation (RSD) of less than 5 % for both intra- and inter-assay precision levels. The recovery rates of MuA in the samples were in the range of 97.6-101.4 % with a RSD of less than 5 %. The developed ELISA exhibited a good correlation value (R 2 = 0.994) with the standard high-performance liquid chromatography method in the crude extracts of plant samples. These results suggest that the developed ELISA method using PAb against MuA can be applied to determine MuA content with high specificity, rapidity, and simplicity in mulberry samples. The developed ELISA method described could prove to be useful as an analytical tool for quality control of mulberry and their products.
AB - An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against mulberroside A (MuA), a major active component found in the root bark of mulberry (Morus alba L.). MuA was conjugated with the carrier protein bovine serum albumin for immunization to rabbits. The results showed that the antibodies were specific only for MuA and have very low specificity for its aglycone, oxyresveratrol. The ELISA assay was suitable for quantitating MuA in the range of 0.17-15.62 μg/mL with a relative standard deviation (RSD) of less than 5 % for both intra- and inter-assay precision levels. The recovery rates of MuA in the samples were in the range of 97.6-101.4 % with a RSD of less than 5 %. The developed ELISA exhibited a good correlation value (R 2 = 0.994) with the standard high-performance liquid chromatography method in the crude extracts of plant samples. These results suggest that the developed ELISA method using PAb against MuA can be applied to determine MuA content with high specificity, rapidity, and simplicity in mulberry samples. The developed ELISA method described could prove to be useful as an analytical tool for quality control of mulberry and their products.
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U2 - 10.1007/s12161-013-9598-4
DO - 10.1007/s12161-013-9598-4
M3 - Article
AN - SCOPUS:84891633041
SN - 1936-9751
VL - 7
SP - 58
EP - 63
JO - Food Analytical Methods
JF - Food Analytical Methods
IS - 1
ER -