Abstract
Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.
| Original language | English |
|---|---|
| Pages (from-to) | 31-36 |
| Number of pages | 6 |
| Journal | Journal of Natural Medicines |
| Volume | 65 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2011 |
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All Science Journal Classification (ASJC) codes
- Organic Chemistry
- Drug Discovery
- Pharmaceutical Science
- Complementary and alternative medicine
Cite this
Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein. / Pongkitwitoon, Benyakarn; Sakamoto, Seiichi; Tanaka, Hiroyuki; Tsuchihashi, Ryota; Kinjo, Junei; Morimoto, Satoshi; Putalun, Waraporn.
In: Journal of Natural Medicines, Vol. 65, No. 1, 01.01.2011, p. 31-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein
AU - Pongkitwitoon, Benyakarn
AU - Sakamoto, Seiichi
AU - Tanaka, Hiroyuki
AU - Tsuchihashi, Ryota
AU - Kinjo, Junei
AU - Morimoto, Satoshi
AU - Putalun, Waraporn
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.
AB - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.
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UR - http://www.scopus.com/inward/citedby.url?scp=78651387053&partnerID=8YFLogxK
U2 - 10.1007/s11418-010-0448-z
DO - 10.1007/s11418-010-0448-z
M3 - Article
C2 - 20658197
AN - SCOPUS:78651387053
VL - 65
SP - 31
EP - 36
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
SN - 1340-3443
IS - 1
ER -