Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Benyakarn Pongkitwitoon; Seiichi Sakamoto; Hiroyuki Tanaka; Ryota Tsuchihashi; Junei Kinjo; Satoshi Morimoto; Waraporn Putalun

doi:10.1007/s11418-010-0448-z

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Benyakarn Pongkitwitoon, Seiichi Sakamoto, Hiroyuki Tanaka, Ryota Tsuchihashi, Junei Kinjo, Satoshi Morimoto, Waraporn Putalun

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

Original language	English
Pages (from-to)	31-36
Number of pages	6
Journal	Journal of Natural Medicines
Volume	65
Issue number	1
DOIs	https://doi.org/10.1007/s11418-010-0448-z
Publication status	Published - Jan 1 2011

All Science Journal Classification (ASJC) codes

Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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Pongkitwitoon, B., Sakamoto, S., Tanaka, H., Tsuchihashi, R., Kinjo, J., Morimoto, S., & Putalun, W. (2011). Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein. Journal of Natural Medicines, 65(1), 31-36. https://doi.org/10.1007/s11418-010-0448-z

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title = "Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein",

abstract = "Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.",

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AU - Pongkitwitoon, Benyakarn

AU - Sakamoto, Seiichi

AU - Tanaka, Hiroyuki

AU - Tsuchihashi, Ryota

AU - Kinjo, Junei

AU - Morimoto, Satoshi

AU - Putalun, Waraporn

PY - 2011/1/1

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