Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Benyakarn Pongkitwitoon, Seiichi Sakamoto, Hiroyuki Tanaka, Ryota Tsuchihashi, Junei Kinjo, Satoshi Morimoto, Waraporn Putalun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalJournal of Natural Medicines
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

Fingerprint

Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
Enzymes
Antibodies
Pueraria
Medicinal Plants
Bovine Serum Albumin
daidzein
puerarin
Antibody Formation
Estrogens
Rabbits

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

Cite this

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein. / Pongkitwitoon, Benyakarn; Sakamoto, Seiichi; Tanaka, Hiroyuki; Tsuchihashi, Ryota; Kinjo, Junei; Morimoto, Satoshi; Putalun, Waraporn.

In: Journal of Natural Medicines, Vol. 65, No. 1, 01.01.2011, p. 31-36.

Research output: Contribution to journalArticle

Pongkitwitoon, Benyakarn ; Sakamoto, Seiichi ; Tanaka, Hiroyuki ; Tsuchihashi, Ryota ; Kinjo, Junei ; Morimoto, Satoshi ; Putalun, Waraporn. / Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein. In: Journal of Natural Medicines. 2011 ; Vol. 65, No. 1. pp. 31-36.
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