Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody

Madan Kumar Paudel, Seiichi Sakamoto, Le Van Huy, Hiroyuki Tanaka, Tomofumi Miyamoto, Akihito Takano, Satoshi Morimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn–bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.

Original languageEnglish
Pages (from-to)457-470
Number of pages14
JournalJournal of Immunoassay and Immunochemistry
Volume38
Issue number5
DOIs
Publication statusPublished - Sep 3 2017

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology

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