TY - JOUR
T1 - Development of an immunochromatographic strip incorporating anti-mitragynine monoclonal antibody conjugated to colloidal gold for kratom alkaloids detection
AU - Limsuwanchote, Supattra
AU - Putalun, Waraporn
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
AU - Keawpradub, Niwat
AU - Wungsintaweekul, Juraithip
N1 - Funding Information:
This research was financially supported by the National Research Council of Thailand (Grant No. PHA560010S) and the PSU budget revenue (Grant No. PHA580949S). The authors are also grateful to the Prince of Songkla University for granting a postdoctoral fellowship to S. Limsuwanchote. Authors thank Prof Dr Allan Coombes for valuable discussion and Mr Trevor Pearson for his assistance with the English.
PY - 2018/7
Y1 - 2018/7
N2 - A lateral flow-based immunochromatographic strip was developed for the rapid detection of mitragynine (MG), a dominant alkaloid found in the leaves of kratom. Monoclonal antibody (mAb) against MG (anti-MG mAb) was conjugated to colloidal gold and used as a recognition probe. MG-ovalbumin conjugate (MG-OVA) and goat anti-mouse IgG were immobilized on the strip to produce a test zone and control zone, respectively. Based on the principle of a competitive assay, MG in a test sample competed with MG-OVA resident in the test zone to bind with colloidal gold-anti-MG mAb, resulting in an inverse relation of color intensity at the test zone and MG amount. The limit of detection (LOD) of the immunochromatographic strip is determined at 1 mg/mL of MG by visual assessment and 0.60 mg/mL by Image J analysis. The developed immunochromatographic strip can determine MG in kratom cocktails and kratom leaf samples. It could serve as a rapid and simple diagnostic kit for the detection of MG in kratom samples.
AB - A lateral flow-based immunochromatographic strip was developed for the rapid detection of mitragynine (MG), a dominant alkaloid found in the leaves of kratom. Monoclonal antibody (mAb) against MG (anti-MG mAb) was conjugated to colloidal gold and used as a recognition probe. MG-ovalbumin conjugate (MG-OVA) and goat anti-mouse IgG were immobilized on the strip to produce a test zone and control zone, respectively. Based on the principle of a competitive assay, MG in a test sample competed with MG-OVA resident in the test zone to bind with colloidal gold-anti-MG mAb, resulting in an inverse relation of color intensity at the test zone and MG amount. The limit of detection (LOD) of the immunochromatographic strip is determined at 1 mg/mL of MG by visual assessment and 0.60 mg/mL by Image J analysis. The developed immunochromatographic strip can determine MG in kratom cocktails and kratom leaf samples. It could serve as a rapid and simple diagnostic kit for the detection of MG in kratom samples.
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U2 - 10.1002/dta.2354
DO - 10.1002/dta.2354
M3 - Article
AN - SCOPUS:85041140149
SN - 1942-7603
VL - 10
SP - 1168
EP - 1175
JO - Drug Testing and Analysis
JF - Drug Testing and Analysis
IS - 7
ER -