Abstract
Harringtonine (HT) is a natural compound, which is mainly produced by the genus Cephalotaxus, and has been clinically utilized in China for the treatment of acute leukemia and lymphoma. However, the amounts of HT in the Cephalotaxus species are very small; therefore, plant tissue cultures have been focused upon to enhance HT production. Qualitative/quantitative methods for HT detection are required to screen superior cell lines. We developed a one-step indirect competitive immunochromatographic assay (ICA) using colloidal gold nanoparticles conjugated with highly specific monoclonal antibodies against HT (MAb 1D2) for simple, rapid, and sensitive detection of HT in plant samples. This ICA can be completed in 15 min after dipping the strip into analytes with a limit of detection of ∼313 ng/mL. In developed ICA, fiber pad which is usually used for conventional ICA, was not used to shorten the time for preparing chromatographic strip, resulting in a decrease in the volume of valuable analytes (20 μL). Considering simplicity, rapidity, and sensitivity of the developed ICA, this study could be applied to a fieldwork study for finding new natural resources containing HT.
| Original language | English |
|---|---|
| Pages (from-to) | 150-154 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 1048 |
| DOIs | |
| Publication status | Published - Mar 24 2017 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Cell Biology
Cite this
Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine. / Sakamoto, Seiichi; Yusakul, Gorawit; Nuntawong, Poomraphie; Kitisripanya, Tharita; Putalun, Waraporn; Miyamoto, Tomofumi; Tanaka, Hiroyuki; Morimoto, Satoshi.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1048, 24.03.2017, p. 150-154.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine
AU - Sakamoto, Seiichi
AU - Yusakul, Gorawit
AU - Nuntawong, Poomraphie
AU - Kitisripanya, Tharita
AU - Putalun, Waraporn
AU - Miyamoto, Tomofumi
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
PY - 2017/3/24
Y1 - 2017/3/24
N2 - Harringtonine (HT) is a natural compound, which is mainly produced by the genus Cephalotaxus, and has been clinically utilized in China for the treatment of acute leukemia and lymphoma. However, the amounts of HT in the Cephalotaxus species are very small; therefore, plant tissue cultures have been focused upon to enhance HT production. Qualitative/quantitative methods for HT detection are required to screen superior cell lines. We developed a one-step indirect competitive immunochromatographic assay (ICA) using colloidal gold nanoparticles conjugated with highly specific monoclonal antibodies against HT (MAb 1D2) for simple, rapid, and sensitive detection of HT in plant samples. This ICA can be completed in 15 min after dipping the strip into analytes with a limit of detection of ∼313 ng/mL. In developed ICA, fiber pad which is usually used for conventional ICA, was not used to shorten the time for preparing chromatographic strip, resulting in a decrease in the volume of valuable analytes (20 μL). Considering simplicity, rapidity, and sensitivity of the developed ICA, this study could be applied to a fieldwork study for finding new natural resources containing HT.
AB - Harringtonine (HT) is a natural compound, which is mainly produced by the genus Cephalotaxus, and has been clinically utilized in China for the treatment of acute leukemia and lymphoma. However, the amounts of HT in the Cephalotaxus species are very small; therefore, plant tissue cultures have been focused upon to enhance HT production. Qualitative/quantitative methods for HT detection are required to screen superior cell lines. We developed a one-step indirect competitive immunochromatographic assay (ICA) using colloidal gold nanoparticles conjugated with highly specific monoclonal antibodies against HT (MAb 1D2) for simple, rapid, and sensitive detection of HT in plant samples. This ICA can be completed in 15 min after dipping the strip into analytes with a limit of detection of ∼313 ng/mL. In developed ICA, fiber pad which is usually used for conventional ICA, was not used to shorten the time for preparing chromatographic strip, resulting in a decrease in the volume of valuable analytes (20 μL). Considering simplicity, rapidity, and sensitivity of the developed ICA, this study could be applied to a fieldwork study for finding new natural resources containing HT.
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UR - http://www.scopus.com/inward/citedby.url?scp=85013411064&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2017.01.032
DO - 10.1016/j.jchromb.2017.01.032
M3 - Article
C2 - 28237091
AN - SCOPUS:85013411064
VL - 1048
SP - 150
EP - 154
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
ER -