Abstract
A sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against asiaticoside (AS), one of the triterpenoid saponins found in Centella asiatica (Linn). AS-bovine serum albumin conjugate was immunized to rabbits for producing PAb. The results showed that the antibodies were specific only for AS and very low specific for madecassoside whose basic skeleton is almost the same as AS. The range of the assay extends from 0. 05-25 μg/ml of AS. A good correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of AS in the crude extracts of plant samples. In addition, the products containing C. asiatica in various preparations were determined AS content by competitive ELISA. The results showed that the product from tea bag preparation gave the highest yield of AS content (35. 59 mg/g dry wt) comparing to other preparations. In order to evaluate the matrix effect of the serum for AS immunoassay, the standard curves of AS in different media were observed. Standard curve of the serum was similar to the water media and both curves showed the measurement range of 0. 20-6. 25 μg/ml. The developed ELISA method can be used for quality assessment of C. asiatica and their products including AS detection in serum samples.
| Original language | English |
|---|---|
| Pages (from-to) | 1320-1327 |
| Number of pages | 8 |
| Journal | Food Analytical Methods |
| Volume | 5 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2012 |
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All Science Journal Classification (ASJC) codes
- Food Science
- Analytical Chemistry
- Applied Microbiology and Biotechnology
- Safety, Risk, Reliability and Quality
- Safety Research
Cite this
Development of Anti-Asiaticoside Polyclonal Antibody-Based Immunoassay and Applications for Centella asiatica Products and Human Serum. / Tassanawat, Patcharin; Putalun, Waraporn; Komaikul, Jukrapun; Sritularak, Boonchoo; Juengwatanatrakul, Thaweesak; Tanaka, Hiroyuki.
In: Food Analytical Methods, Vol. 5, No. 6, 01.12.2012, p. 1320-1327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of Anti-Asiaticoside Polyclonal Antibody-Based Immunoassay and Applications for Centella asiatica Products and Human Serum
AU - Tassanawat, Patcharin
AU - Putalun, Waraporn
AU - Komaikul, Jukrapun
AU - Sritularak, Boonchoo
AU - Juengwatanatrakul, Thaweesak
AU - Tanaka, Hiroyuki
PY - 2012/12/1
Y1 - 2012/12/1
N2 - A sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against asiaticoside (AS), one of the triterpenoid saponins found in Centella asiatica (Linn). AS-bovine serum albumin conjugate was immunized to rabbits for producing PAb. The results showed that the antibodies were specific only for AS and very low specific for madecassoside whose basic skeleton is almost the same as AS. The range of the assay extends from 0. 05-25 μg/ml of AS. A good correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of AS in the crude extracts of plant samples. In addition, the products containing C. asiatica in various preparations were determined AS content by competitive ELISA. The results showed that the product from tea bag preparation gave the highest yield of AS content (35. 59 mg/g dry wt) comparing to other preparations. In order to evaluate the matrix effect of the serum for AS immunoassay, the standard curves of AS in different media were observed. Standard curve of the serum was similar to the water media and both curves showed the measurement range of 0. 20-6. 25 μg/ml. The developed ELISA method can be used for quality assessment of C. asiatica and their products including AS detection in serum samples.
AB - A sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against asiaticoside (AS), one of the triterpenoid saponins found in Centella asiatica (Linn). AS-bovine serum albumin conjugate was immunized to rabbits for producing PAb. The results showed that the antibodies were specific only for AS and very low specific for madecassoside whose basic skeleton is almost the same as AS. The range of the assay extends from 0. 05-25 μg/ml of AS. A good correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of AS in the crude extracts of plant samples. In addition, the products containing C. asiatica in various preparations were determined AS content by competitive ELISA. The results showed that the product from tea bag preparation gave the highest yield of AS content (35. 59 mg/g dry wt) comparing to other preparations. In order to evaluate the matrix effect of the serum for AS immunoassay, the standard curves of AS in different media were observed. Standard curve of the serum was similar to the water media and both curves showed the measurement range of 0. 20-6. 25 μg/ml. The developed ELISA method can be used for quality assessment of C. asiatica and their products including AS detection in serum samples.
UR - http://www.scopus.com/inward/record.url?scp=84867682132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867682132&partnerID=8YFLogxK
U2 - 10.1007/s12161-012-9380-z
DO - 10.1007/s12161-012-9380-z
M3 - Article
AN - SCOPUS:84867682132
VL - 5
SP - 1320
EP - 1327
JO - Food Analytical Methods
JF - Food Analytical Methods
SN - 1936-9751
IS - 6
ER -