Development of Anti-Asiaticoside Polyclonal Antibody-Based Immunoassay and Applications for Centella asiatica Products and Human Serum

Patcharin Tassanawat, Waraporn Putalun, Jukrapun Komaikul, Boonchoo Sritularak, Thaweesak Juengwatanatrakul, Hiroyuki Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against asiaticoside (AS), one of the triterpenoid saponins found in Centella asiatica (Linn). AS-bovine serum albumin conjugate was immunized to rabbits for producing PAb. The results showed that the antibodies were specific only for AS and very low specific for madecassoside whose basic skeleton is almost the same as AS. The range of the assay extends from 0. 05-25 μg/ml of AS. A good correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of AS in the crude extracts of plant samples. In addition, the products containing C. asiatica in various preparations were determined AS content by competitive ELISA. The results showed that the product from tea bag preparation gave the highest yield of AS content (35. 59 mg/g dry wt) comparing to other preparations. In order to evaluate the matrix effect of the serum for AS immunoassay, the standard curves of AS in different media were observed. Standard curve of the serum was similar to the water media and both curves showed the measurement range of 0. 20-6. 25 μg/ml. The developed ELISA method can be used for quality assessment of C. asiatica and their products including AS detection in serum samples.

Original languageEnglish
Pages (from-to)1320-1327
Number of pages8
JournalFood Analytical Methods
Volume5
Issue number6
DOIs
Publication statusPublished - Dec 1 2012

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Centella
Centella asiatica
immunoassays
polyclonal antibodies
Immunoassay
Antibodies
Assays
enzyme-linked immunosorbent assay
Enzymes
Serum
Immunosorbents
triterpenoid saponins
Enzyme-Linked Immunosorbent Assay
bovine serum albumin
plant extracts
tea
skeleton
bags
High performance liquid chromatography
water

All Science Journal Classification (ASJC) codes

  • Food Science
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

Development of Anti-Asiaticoside Polyclonal Antibody-Based Immunoassay and Applications for Centella asiatica Products and Human Serum. / Tassanawat, Patcharin; Putalun, Waraporn; Komaikul, Jukrapun; Sritularak, Boonchoo; Juengwatanatrakul, Thaweesak; Tanaka, Hiroyuki.

In: Food Analytical Methods, Vol. 5, No. 6, 01.12.2012, p. 1320-1327.

Research output: Contribution to journalArticle

Tassanawat, Patcharin ; Putalun, Waraporn ; Komaikul, Jukrapun ; Sritularak, Boonchoo ; Juengwatanatrakul, Thaweesak ; Tanaka, Hiroyuki. / Development of Anti-Asiaticoside Polyclonal Antibody-Based Immunoassay and Applications for Centella asiatica Products and Human Serum. In: Food Analytical Methods. 2012 ; Vol. 5, No. 6. pp. 1320-1327.
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