Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp

Tomonori Somamoto, Shinpei Maruyama, Takahiro Nagasawa, Miki Nakao, Atsushi Sato, Hajime Hatta, Mitsuru Sato, Yukie Murakami-Yamaguchi, Kumiko Kizu-Mori, Yuki Hirakawa, Hiroshi Narita

Research output: Contribution to journalArticle

Abstract

To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromo-nads.

Original languageEnglish
Pages (from-to)36-39
Number of pages4
JournalFish Pathology
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

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koi
Aeromonas salmonicida
carp
antibody
monoclonal antibodies
Paralichthys olivaceus
agar
bacterium
bacteria

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Animal Science and Zoology

Cite this

Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp. / Somamoto, Tomonori; Maruyama, Shinpei; Nagasawa, Takahiro; Nakao, Miki; Sato, Atsushi; Hatta, Hajime; Sato, Mitsuru; Murakami-Yamaguchi, Yukie; Kizu-Mori, Kumiko; Hirakawa, Yuki; Narita, Hiroshi.

In: Fish Pathology, Vol. 53, No. 1, 01.01.2018, p. 36-39.

Research output: Contribution to journalArticle

Somamoto, T, Maruyama, S, Nagasawa, T, Nakao, M, Sato, A, Hatta, H, Sato, M, Murakami-Yamaguchi, Y, Kizu-Mori, K, Hirakawa, Y & Narita, H 2018, 'Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp', Fish Pathology, vol. 53, no. 1, pp. 36-39. https://doi.org/10.3147/jsfp.53.36
Somamoto, Tomonori ; Maruyama, Shinpei ; Nagasawa, Takahiro ; Nakao, Miki ; Sato, Atsushi ; Hatta, Hajime ; Sato, Mitsuru ; Murakami-Yamaguchi, Yukie ; Kizu-Mori, Kumiko ; Hirakawa, Yuki ; Narita, Hiroshi. / Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of “new ulcer disease⇝ in koi carp. In: Fish Pathology. 2018 ; Vol. 53, No. 1. pp. 36-39.
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