Abstract
We tried to establish an assay system for screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesive cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesive cells, lipopolysaccharides, pokeweed mitogen, and phytohemagglutinin stimulated the production of IgA, IgG, and IgM in a class-dependent manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens. When mouse splenocytes were cultured with milk proteins in the absence of adhesive cells, lactoferrin, β-lactoglobulin, α-casein, and β-casein stimulated IgA and IgG production. Adhesive cells increased IgA production of splenocytes stimulated with milk proteins, especially. These results suggest that the assay system is useful for assessment of Ig production-regulating factors.
| Original language | English |
|---|---|
| Pages (from-to) | 143-149 |
| Number of pages | 7 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 65 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2001 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes. / Takasugi, Mikako; Tamura, Yuki; Tachibana, Hirofumi; Sugano, Michihiro; Yamada, Koji.
In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 1, 01.01.2001, p. 143-149.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes
AU - Takasugi, Mikako
AU - Tamura, Yuki
AU - Tachibana, Hirofumi
AU - Sugano, Michihiro
AU - Yamada, Koji
PY - 2001/1/1
Y1 - 2001/1/1
N2 - We tried to establish an assay system for screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesive cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesive cells, lipopolysaccharides, pokeweed mitogen, and phytohemagglutinin stimulated the production of IgA, IgG, and IgM in a class-dependent manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens. When mouse splenocytes were cultured with milk proteins in the absence of adhesive cells, lactoferrin, β-lactoglobulin, α-casein, and β-casein stimulated IgA and IgG production. Adhesive cells increased IgA production of splenocytes stimulated with milk proteins, especially. These results suggest that the assay system is useful for assessment of Ig production-regulating factors.
AB - We tried to establish an assay system for screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesive cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesive cells, lipopolysaccharides, pokeweed mitogen, and phytohemagglutinin stimulated the production of IgA, IgG, and IgM in a class-dependent manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens. When mouse splenocytes were cultured with milk proteins in the absence of adhesive cells, lactoferrin, β-lactoglobulin, α-casein, and β-casein stimulated IgA and IgG production. Adhesive cells increased IgA production of splenocytes stimulated with milk proteins, especially. These results suggest that the assay system is useful for assessment of Ig production-regulating factors.
UR - http://www.scopus.com/inward/record.url?scp=0035221114&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035221114&partnerID=8YFLogxK
U2 - 10.1271/bbb.65.143
DO - 10.1271/bbb.65.143
M3 - Article
C2 - 11272818
AN - SCOPUS:0035221114
VL - 65
SP - 143
EP - 149
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 1
ER -