Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes

Mikako Takasugi, Yuki Tamura, Hirofumi Tachibana, Michihiro Sugano, Koji Yamada

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We tried to establish an assay system for screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesive cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesive cells, lipopolysaccharides, pokeweed mitogen, and phytohemagglutinin stimulated the production of IgA, IgG, and IgM in a class-dependent manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens. When mouse splenocytes were cultured with milk proteins in the absence of adhesive cells, lactoferrin, β-lactoglobulin, α-casein, and β-casein stimulated IgA and IgG production. Adhesive cells increased IgA production of splenocytes stimulated with milk proteins, especially. These results suggest that the assay system is useful for assessment of Ig production-regulating factors.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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