Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading

Fukuda Keisuke, Omata Seiji, Yoshinori Sawae

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, chondrocytes isolated from bovine cartilage tissue were seeded in agarose gel and resultant chondrocyte- agarose constructs, a well-established experimental model to examine the effect of mechanical loadings on the chondrocyte metabolism, were cultured with a traction loading on the construct surface to examine its effect on the regeneration of the cartilaginous tissue by chondrocytes. Customdesigned mechanical loading equipment was developed to apply the traction loading on the upper surface of constructs being cultured in the CO2 incubator. After 2 or 3 weeks culture, quantities of glycosaminoglycan (GAG) molecules that proteoglycan, and type II collagen were determined, and immunofluorescent staining of keratin sulfate, a type of GAG, and type II collagen was performed to verify the chondrocyte biosynthesis of extra cellular matrix (ECM) and characterize the structure of elaborated cartilaginous tissue by confocal laser scanning microscopy (CLSM). Results indicated that the traction loading enhance ECM biosynthesis in the surface region of constructs and collagen rich layer covered with GAG rich superficial layer was formed in the articulation surface. Results of quantification for ECM molecules indicated that the production of type II collagen and GAG was more significant outside the slide track compared with inside the slide track.

Original languageEnglish
Title of host publicationThe 15th International Conference on Biomedical Engineering, ICBME 2013
PublisherSpringer Verlag
Pages263-266
Number of pages4
Volume43
ISBN (Electronic)9783319029122
DOIs
Publication statusPublished - 2014
Event15th International Conference on Biomedical Engineering, ICBME 2013 - Singapore, Singapore
Duration: Dec 4 2013Dec 7 2013

Other

Other15th International Conference on Biomedical Engineering, ICBME 2013
CountrySingapore
CitySingapore
Period12/4/1312/7/13

Fingerprint

Glycosaminoglycans
Collagen
Sepharose
Collagen Type II
Tissue
Biosynthesis
Keratin
Molecules
Cartilage
Proteoglycans
Keratins
Metabolism
Sulfates
Microscopic examination
Gels
Scanning
Lasers

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Bioengineering

Cite this

Keisuke, F., Seiji, O., & Sawae, Y. (2014). Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading. In The 15th International Conference on Biomedical Engineering, ICBME 2013 (Vol. 43, pp. 263-266). Springer Verlag. https://doi.org/10.1007/978-3-319-02913-9_67

Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading. / Keisuke, Fukuda; Seiji, Omata; Sawae, Yoshinori.

The 15th International Conference on Biomedical Engineering, ICBME 2013. Vol. 43 Springer Verlag, 2014. p. 263-266.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Keisuke, F, Seiji, O & Sawae, Y 2014, Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading. in The 15th International Conference on Biomedical Engineering, ICBME 2013. vol. 43, Springer Verlag, pp. 263-266, 15th International Conference on Biomedical Engineering, ICBME 2013, Singapore, Singapore, 12/4/13. https://doi.org/10.1007/978-3-319-02913-9_67
Keisuke F, Seiji O, Sawae Y. Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading. In The 15th International Conference on Biomedical Engineering, ICBME 2013. Vol. 43. Springer Verlag. 2014. p. 263-266 https://doi.org/10.1007/978-3-319-02913-9_67
Keisuke, Fukuda ; Seiji, Omata ; Sawae, Yoshinori. / Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading. The 15th International Conference on Biomedical Engineering, ICBME 2013. Vol. 43 Springer Verlag, 2014. pp. 263-266
@inproceedings{d80aed58dc054ec7be85913c083cdba0,
title = "Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading",
abstract = "In this study, chondrocytes isolated from bovine cartilage tissue were seeded in agarose gel and resultant chondrocyte- agarose constructs, a well-established experimental model to examine the effect of mechanical loadings on the chondrocyte metabolism, were cultured with a traction loading on the construct surface to examine its effect on the regeneration of the cartilaginous tissue by chondrocytes. Customdesigned mechanical loading equipment was developed to apply the traction loading on the upper surface of constructs being cultured in the CO2 incubator. After 2 or 3 weeks culture, quantities of glycosaminoglycan (GAG) molecules that proteoglycan, and type II collagen were determined, and immunofluorescent staining of keratin sulfate, a type of GAG, and type II collagen was performed to verify the chondrocyte biosynthesis of extra cellular matrix (ECM) and characterize the structure of elaborated cartilaginous tissue by confocal laser scanning microscopy (CLSM). Results indicated that the traction loading enhance ECM biosynthesis in the surface region of constructs and collagen rich layer covered with GAG rich superficial layer was formed in the articulation surface. Results of quantification for ECM molecules indicated that the production of type II collagen and GAG was more significant outside the slide track compared with inside the slide track.",
author = "Fukuda Keisuke and Omata Seiji and Yoshinori Sawae",
year = "2014",
doi = "10.1007/978-3-319-02913-9_67",
language = "English",
volume = "43",
pages = "263--266",
booktitle = "The 15th International Conference on Biomedical Engineering, ICBME 2013",
publisher = "Springer Verlag",
address = "Germany",

}

TY - GEN

T1 - Development of cartilaginous tissue in chondrocyte-agarose construct cultured under traction loading

AU - Keisuke, Fukuda

AU - Seiji, Omata

AU - Sawae, Yoshinori

PY - 2014

Y1 - 2014

N2 - In this study, chondrocytes isolated from bovine cartilage tissue were seeded in agarose gel and resultant chondrocyte- agarose constructs, a well-established experimental model to examine the effect of mechanical loadings on the chondrocyte metabolism, were cultured with a traction loading on the construct surface to examine its effect on the regeneration of the cartilaginous tissue by chondrocytes. Customdesigned mechanical loading equipment was developed to apply the traction loading on the upper surface of constructs being cultured in the CO2 incubator. After 2 or 3 weeks culture, quantities of glycosaminoglycan (GAG) molecules that proteoglycan, and type II collagen were determined, and immunofluorescent staining of keratin sulfate, a type of GAG, and type II collagen was performed to verify the chondrocyte biosynthesis of extra cellular matrix (ECM) and characterize the structure of elaborated cartilaginous tissue by confocal laser scanning microscopy (CLSM). Results indicated that the traction loading enhance ECM biosynthesis in the surface region of constructs and collagen rich layer covered with GAG rich superficial layer was formed in the articulation surface. Results of quantification for ECM molecules indicated that the production of type II collagen and GAG was more significant outside the slide track compared with inside the slide track.

AB - In this study, chondrocytes isolated from bovine cartilage tissue were seeded in agarose gel and resultant chondrocyte- agarose constructs, a well-established experimental model to examine the effect of mechanical loadings on the chondrocyte metabolism, were cultured with a traction loading on the construct surface to examine its effect on the regeneration of the cartilaginous tissue by chondrocytes. Customdesigned mechanical loading equipment was developed to apply the traction loading on the upper surface of constructs being cultured in the CO2 incubator. After 2 or 3 weeks culture, quantities of glycosaminoglycan (GAG) molecules that proteoglycan, and type II collagen were determined, and immunofluorescent staining of keratin sulfate, a type of GAG, and type II collagen was performed to verify the chondrocyte biosynthesis of extra cellular matrix (ECM) and characterize the structure of elaborated cartilaginous tissue by confocal laser scanning microscopy (CLSM). Results indicated that the traction loading enhance ECM biosynthesis in the surface region of constructs and collagen rich layer covered with GAG rich superficial layer was formed in the articulation surface. Results of quantification for ECM molecules indicated that the production of type II collagen and GAG was more significant outside the slide track compared with inside the slide track.

UR - http://www.scopus.com/inward/record.url?scp=84928234777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928234777&partnerID=8YFLogxK

U2 - 10.1007/978-3-319-02913-9_67

DO - 10.1007/978-3-319-02913-9_67

M3 - Conference contribution

AN - SCOPUS:84928234777

VL - 43

SP - 263

EP - 266

BT - The 15th International Conference on Biomedical Engineering, ICBME 2013

PB - Springer Verlag

ER -