Abstract
In vitro rapid and quantitative cell-based assay is demanded to verify the efficacy prediction of cancer drugs since a cancer patient may have unconventional aspects of tumor development. Here, we show the rapid and non-label quantitative verifying method and instrumentation of apoptosis for cell cycle-arrest type cancer drugs (Roscovitine and D-allose) by reaction analysis of living liver cancer cells cultured on a sensor chip with a newly developed high precision (50 ndeg s-1 average fluctuation) surface plasmon resonance (SPR) sensor. The time-course cell reaction as the SPR angle change rate for 10 min from 30 min cell culture with a drug was significantly related to cell viability. By the simultaneous detection of differential SPR angle change and fluorescence by specific probes using the new instrument, the SPR angle was related to the nano-order potential decrease in inner mitochondrial membrane potential. The results obtained are universally valid for the cell cycle-arrest type cancer drugs, which mediate apoptosis through different cell-signaling pathways, by a liver cancer cell line of Hep G2 (P≤0.001). This system towards the application to evaluate personal therapeutic potentials of drugs using cancer cells from patients in clinical use.
| Original language | English |
|---|---|
| Title of host publication | Biophotonics |
| Subtitle of host publication | Photonic Solutions for Better Health Care |
| Volume | 6991 |
| DOIs | |
| Publication status | Published - Jun 18 2008 |
| Event | Biophotonics: Photonic Solutions for Better Health Care - Strasbourg, France Duration: Apr 8 2008 → Apr 10 2008 |
Other
| Other | Biophotonics: Photonic Solutions for Better Health Care |
|---|---|
| Country | France |
| City | Strasbourg |
| Period | 4/8/08 → 4/10/08 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
Cite this
Development of cell-based quantitative evaluation method for cell cycle-arrest type cancer drugs for apoptosis by high precision surface plasmon resonance sensor. / Ona, Toshihiro; Nishijima, Hiroshi; Kosaihira, Atsushi; Shibata, Junko.
Biophotonics: Photonic Solutions for Better Health Care. Vol. 6991 2008. 69910R.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Development of cell-based quantitative evaluation method for cell cycle-arrest type cancer drugs for apoptosis by high precision surface plasmon resonance sensor
AU - Ona, Toshihiro
AU - Nishijima, Hiroshi
AU - Kosaihira, Atsushi
AU - Shibata, Junko
PY - 2008/6/18
Y1 - 2008/6/18
N2 - In vitro rapid and quantitative cell-based assay is demanded to verify the efficacy prediction of cancer drugs since a cancer patient may have unconventional aspects of tumor development. Here, we show the rapid and non-label quantitative verifying method and instrumentation of apoptosis for cell cycle-arrest type cancer drugs (Roscovitine and D-allose) by reaction analysis of living liver cancer cells cultured on a sensor chip with a newly developed high precision (50 ndeg s-1 average fluctuation) surface plasmon resonance (SPR) sensor. The time-course cell reaction as the SPR angle change rate for 10 min from 30 min cell culture with a drug was significantly related to cell viability. By the simultaneous detection of differential SPR angle change and fluorescence by specific probes using the new instrument, the SPR angle was related to the nano-order potential decrease in inner mitochondrial membrane potential. The results obtained are universally valid for the cell cycle-arrest type cancer drugs, which mediate apoptosis through different cell-signaling pathways, by a liver cancer cell line of Hep G2 (P≤0.001). This system towards the application to evaluate personal therapeutic potentials of drugs using cancer cells from patients in clinical use.
AB - In vitro rapid and quantitative cell-based assay is demanded to verify the efficacy prediction of cancer drugs since a cancer patient may have unconventional aspects of tumor development. Here, we show the rapid and non-label quantitative verifying method and instrumentation of apoptosis for cell cycle-arrest type cancer drugs (Roscovitine and D-allose) by reaction analysis of living liver cancer cells cultured on a sensor chip with a newly developed high precision (50 ndeg s-1 average fluctuation) surface plasmon resonance (SPR) sensor. The time-course cell reaction as the SPR angle change rate for 10 min from 30 min cell culture with a drug was significantly related to cell viability. By the simultaneous detection of differential SPR angle change and fluorescence by specific probes using the new instrument, the SPR angle was related to the nano-order potential decrease in inner mitochondrial membrane potential. The results obtained are universally valid for the cell cycle-arrest type cancer drugs, which mediate apoptosis through different cell-signaling pathways, by a liver cancer cell line of Hep G2 (P≤0.001). This system towards the application to evaluate personal therapeutic potentials of drugs using cancer cells from patients in clinical use.
UR - http://www.scopus.com/inward/record.url?scp=44949254123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44949254123&partnerID=8YFLogxK
U2 - 10.1117/12.780819
DO - 10.1117/12.780819
M3 - Conference contribution
AN - SCOPUS:44949254123
SN - 9780819471895
VL - 6991
BT - Biophotonics
ER -