Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure

Yuma Yamada, Kentaro Kogure, Yoshio Nakamura, Kaori Inoue, Hidetaka Akita, Fumi Nagatsugi, Shigeki Sasaki, Tetsuya Suhara, Hideyoshi Harashima

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.

Original languageEnglish
Pages (from-to)1939-1942
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number10
DOIs
Publication statusPublished - Oct 1 2005

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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    Yamada, Y., Kogure, K., Nakamura, Y., Inoue, K., Akita, H., Nagatsugi, F., Sasaki, S., Suhara, T., & Harashima, H. (2005). Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure. Biological and Pharmaceutical Bulletin, 28(10), 1939-1942. https://doi.org/10.1248/bpb.28.1939