Development of EST-SSR markers of Ipomoea nil

Tong Ly, Hiroyuki Fukuoka, Asami Otaka, Atsushi Hoshino, Shigeru Iida, Eiji Nitasaka, Nobuyoshi Watanabe, Tsutomu Kuboyama

Research output: Contribution to journalComment/debate

6 Citations (Scopus)

Abstract

Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalBreeding Science
Volume62
Issue number1
DOIs
Publication statusPublished - Mar 26 2012

Fingerprint

Ipomoea nil
Expressed Sequence Tags
expressed sequence tags
Microsatellite Repeats
microsatellite repeats
Genetic Markers
Chromosomes
Genotype
genetic polymorphism
chromosomes
genetic markers
genotype

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Ly, T., Fukuoka, H., Otaka, A., Hoshino, A., Iida, S., Nitasaka, E., ... Kuboyama, T. (2012). Development of EST-SSR markers of Ipomoea nil. Breeding Science, 62(1), 99-104. https://doi.org/10.1270/jsbbs.62.99

Development of EST-SSR markers of Ipomoea nil. / Ly, Tong; Fukuoka, Hiroyuki; Otaka, Asami; Hoshino, Atsushi; Iida, Shigeru; Nitasaka, Eiji; Watanabe, Nobuyoshi; Kuboyama, Tsutomu.

In: Breeding Science, Vol. 62, No. 1, 26.03.2012, p. 99-104.

Research output: Contribution to journalComment/debate

Ly, T, Fukuoka, H, Otaka, A, Hoshino, A, Iida, S, Nitasaka, E, Watanabe, N & Kuboyama, T 2012, 'Development of EST-SSR markers of Ipomoea nil', Breeding Science, vol. 62, no. 1, pp. 99-104. https://doi.org/10.1270/jsbbs.62.99
Ly T, Fukuoka H, Otaka A, Hoshino A, Iida S, Nitasaka E et al. Development of EST-SSR markers of Ipomoea nil. Breeding Science. 2012 Mar 26;62(1):99-104. https://doi.org/10.1270/jsbbs.62.99
Ly, Tong ; Fukuoka, Hiroyuki ; Otaka, Asami ; Hoshino, Atsushi ; Iida, Shigeru ; Nitasaka, Eiji ; Watanabe, Nobuyoshi ; Kuboyama, Tsutomu. / Development of EST-SSR markers of Ipomoea nil. In: Breeding Science. 2012 ; Vol. 62, No. 1. pp. 99-104.
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