Development of functional human embryonic stem cell-derived neurons in mouse brain

Alysson R. Muotri, Kinichi Nakashima, Nicolas Toni, Vladislav M. Sandler, Fred H. Gage

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Human embryonic stem cells are pluripotent entities, theoretically capable of generating a whole-body spectrum of distinct cell types. However, differentiation of these cells has been observed only in culture or during teratoma formation. Our results show that human embryonic stem cells implanted in the brain ventricles of embryonic mice can differentiate into functional neural lineages and generate mature, active human neurons that successfully integrate into the adult mouse forebrain. Moreover, this study reveals the conservation and recognition of common signals for neural differentiation throughout mammalian evolution. The chimeric model will permit the study of human neural development in a live environment, paving the way for the generation of new models of human neurodegenerative and psychiatric diseases. The model also has the potential to speed up the screening process for therapeutic drugs.

Original languageEnglish
Pages (from-to)18644-18648
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number51
DOIs
Publication statusPublished - Dec 20 2005
Externally publishedYes

Fingerprint

Neurons
Teratoma
Brain
Human Development
Prosencephalon
Neurodegenerative Diseases
Psychiatry
Cell Differentiation
Pharmaceutical Preparations
Human Embryonic Stem Cells
Therapeutics

All Science Journal Classification (ASJC) codes

  • General

Cite this

Development of functional human embryonic stem cell-derived neurons in mouse brain. / Muotri, Alysson R.; Nakashima, Kinichi; Toni, Nicolas; Sandler, Vladislav M.; Gage, Fred H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 51, 20.12.2005, p. 18644-18648.

Research output: Contribution to journalArticle

Muotri, Alysson R. ; Nakashima, Kinichi ; Toni, Nicolas ; Sandler, Vladislav M. ; Gage, Fred H. / Development of functional human embryonic stem cell-derived neurons in mouse brain. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 51. pp. 18644-18648.
@article{4526a74b321340258e08fa01a3de14bc,
title = "Development of functional human embryonic stem cell-derived neurons in mouse brain",
abstract = "Human embryonic stem cells are pluripotent entities, theoretically capable of generating a whole-body spectrum of distinct cell types. However, differentiation of these cells has been observed only in culture or during teratoma formation. Our results show that human embryonic stem cells implanted in the brain ventricles of embryonic mice can differentiate into functional neural lineages and generate mature, active human neurons that successfully integrate into the adult mouse forebrain. Moreover, this study reveals the conservation and recognition of common signals for neural differentiation throughout mammalian evolution. The chimeric model will permit the study of human neural development in a live environment, paving the way for the generation of new models of human neurodegenerative and psychiatric diseases. The model also has the potential to speed up the screening process for therapeutic drugs.",
author = "Muotri, {Alysson R.} and Kinichi Nakashima and Nicolas Toni and Sandler, {Vladislav M.} and Gage, {Fred H.}",
year = "2005",
month = "12",
day = "20",
doi = "10.1073/pnas.0509315102",
language = "English",
volume = "102",
pages = "18644--18648",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "51",

}

TY - JOUR

T1 - Development of functional human embryonic stem cell-derived neurons in mouse brain

AU - Muotri, Alysson R.

AU - Nakashima, Kinichi

AU - Toni, Nicolas

AU - Sandler, Vladislav M.

AU - Gage, Fred H.

PY - 2005/12/20

Y1 - 2005/12/20

N2 - Human embryonic stem cells are pluripotent entities, theoretically capable of generating a whole-body spectrum of distinct cell types. However, differentiation of these cells has been observed only in culture or during teratoma formation. Our results show that human embryonic stem cells implanted in the brain ventricles of embryonic mice can differentiate into functional neural lineages and generate mature, active human neurons that successfully integrate into the adult mouse forebrain. Moreover, this study reveals the conservation and recognition of common signals for neural differentiation throughout mammalian evolution. The chimeric model will permit the study of human neural development in a live environment, paving the way for the generation of new models of human neurodegenerative and psychiatric diseases. The model also has the potential to speed up the screening process for therapeutic drugs.

AB - Human embryonic stem cells are pluripotent entities, theoretically capable of generating a whole-body spectrum of distinct cell types. However, differentiation of these cells has been observed only in culture or during teratoma formation. Our results show that human embryonic stem cells implanted in the brain ventricles of embryonic mice can differentiate into functional neural lineages and generate mature, active human neurons that successfully integrate into the adult mouse forebrain. Moreover, this study reveals the conservation and recognition of common signals for neural differentiation throughout mammalian evolution. The chimeric model will permit the study of human neural development in a live environment, paving the way for the generation of new models of human neurodegenerative and psychiatric diseases. The model also has the potential to speed up the screening process for therapeutic drugs.

UR - http://www.scopus.com/inward/record.url?scp=29444439070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29444439070&partnerID=8YFLogxK

U2 - 10.1073/pnas.0509315102

DO - 10.1073/pnas.0509315102

M3 - Article

C2 - 16352714

AN - SCOPUS:29444439070

VL - 102

SP - 18644

EP - 18648

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 51

ER -