Development of growth factor-immobilizable material for hepatocyte transplantation

Yung Te Hou, Hiroyuki Ijima, Nana Shirakigawa, Takayuki Takei, Koei Kawakami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Growth factor (GF)-immobilizable materials were developed as a practical hepatocyte transplantation method for reconstructing a tissue-like structure in liver tissue engineering. Two GF-immobilizable scaffolds, namely single hepatocyte-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam, and hepatocyte spheroid-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam were developed by covalently incorporating heparin into collagen gel, using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/. N-hydroxysuccinimide for hepatocyte transplantation. Seventy percent partial hepatectomy (PH) was performed at the same time after hepatocyte transplantation. Angiogenesis efficiency and viability of transplanted cells are discussed in terms of normalized hemoglobin content, nuclear density and histological observations after transplantation. In summary, the normalized hemoglobin content and viability of transplanted cells were higher in GF-immobilized scaffolds with PH pretreatment than in the other scaffolds with/without PH pretreatment. These materials have the potential for in vivo hepatocyte transplantation, as GFs released from remnant liver were easily incorporated into the heparin-immobilized collagen gel system. These GF-heparin complexes may promote the survival of embedded cells. Furthermore, the transplantation of spheroids promoted increased angiogenesis compared with hepatocytes, and resulted in sufficient vascularization for cell survival.

Original languageEnglish
Pages (from-to)172-181
Number of pages10
JournalBiochemical Engineering Journal
Volume69
DOIs
Publication statusPublished - Dec 15 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

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