Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Masayasu Taki, Shohei Iyoshi, Akio Ojida, Itaru Hamachi, Yukio Yamamoto

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of CuI present in living cells.

Original languageEnglish
Pages (from-to)5938-5939
Number of pages2
JournalJournal of the American Chemical Society
Volume132
Issue number17
DOIs
Publication statusPublished - May 5 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

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