Development of human-derived hemoglobin–albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification

Seiichi Ohta, Kenichiro Hashimoto, Xiaoting Fu, Masamichi Kamihira, Yasuyuki Sakai, Taichi Ito

Research output: Contribution to journalArticle

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Abstract

Novel hemoglobin-based artificial oxygen carriers are necessary in tissue engineering. We developed human hemoglobin (hHb) and albumin (HSA)-based microspheres using Shirasu porous glass (SPG) membrane emulsification. The obtained microspheres had a uniform size with an average diameter of 15.1 μm measured by optical microscope, which is similar to the diameter of human red blood cells (7–8 μm). The loading amount of hHb in the microspheres was 20 wt%, which is similar to that of red blood cells (33 wt%). The hHb–HSA microspheres showed similar oxygen dissociation behavior and methemoglobin formation resistance to native hHb. Incubation with genetically engineered HeLa cells that shows hypoxia-responsive EGFP expression demonstrated efficient oxygen supply from the microspheres. Our study suggests the utility of hHb–HSA microspheres as oxygen carriers for tissue engineering with a low risk of infectious disease.

Original languageEnglish
Pages (from-to)533-539
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume126
Issue number4
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Emulsification
Human Development
Microspheres
Glass
Hemoglobin
Oxygen
Membranes
Hemoglobins
Tissue Engineering
Tissue engineering
Blood
Erythrocytes
Cells
Oxygen supply
Blood Substitutes
Cell Hypoxia
Methemoglobin
HeLa Cells
Communicable Diseases
Albumins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Development of human-derived hemoglobin–albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification. / Ohta, Seiichi; Hashimoto, Kenichiro; Fu, Xiaoting; Kamihira, Masamichi; Sakai, Yasuyuki; Ito, Taichi.

In: Journal of Bioscience and Bioengineering, Vol. 126, No. 4, 01.10.2018, p. 533-539.

Research output: Contribution to journalArticle

Ohta, Seiichi ; Hashimoto, Kenichiro ; Fu, Xiaoting ; Kamihira, Masamichi ; Sakai, Yasuyuki ; Ito, Taichi. / Development of human-derived hemoglobin–albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification. In: Journal of Bioscience and Bioengineering. 2018 ; Vol. 126, No. 4. pp. 533-539.
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