Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection

A. S. Mweene, T. Ito, K. Okazaki, Etsuro Ono, Y. Shimizu, H. Kida

Research output: Contribution to journalArticle

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Abstract

A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 107.0 times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 105.0 times higher than that of ELISA for antibody detection.

Original languageEnglish
Pages (from-to)748-750
Number of pages3
JournalJournal of Clinical Microbiology
Volume34
Issue number3
Publication statusPublished - Feb 26 1996
Externally publishedYes

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Bovine Herpesvirus 1
Herpesviridae Infections
Enzyme-Linked Immunosorbent Assay
Antigens
Polymerase Chain Reaction
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Mweene, A. S., Ito, T., Okazaki, K., Ono, E., Shimizu, Y., & Kida, H. (1996). Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection. Journal of Clinical Microbiology, 34(3), 748-750.

Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection. / Mweene, A. S.; Ito, T.; Okazaki, K.; Ono, Etsuro; Shimizu, Y.; Kida, H.

In: Journal of Clinical Microbiology, Vol. 34, No. 3, 26.02.1996, p. 748-750.

Research output: Contribution to journalArticle

Mweene, AS, Ito, T, Okazaki, K, Ono, E, Shimizu, Y & Kida, H 1996, 'Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection', Journal of Clinical Microbiology, vol. 34, no. 3, pp. 748-750.
Mweene AS, Ito T, Okazaki K, Ono E, Shimizu Y, Kida H. Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection. Journal of Clinical Microbiology. 1996 Feb 26;34(3):748-750.
Mweene, A. S. ; Ito, T. ; Okazaki, K. ; Ono, Etsuro ; Shimizu, Y. ; Kida, H. / Development of immuno-PCR for diagnosis of bovine herpesvirus 1 infection. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 3. pp. 748-750.
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