Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay

Toshihiro Kasama, Yutaka Hasegawa, Haruyasu Kondo, Tsutomu Ozawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have developed a novel immunoassay device which has a long and thin wall structure inside a microchannel, hence the name 'immuno-wall device'. Unreacted antigens and antibodies were completely removed by just immersing the device in a washing buffer for 1 minute. The long structure also allowed us to analyze fluorescence intensity by using inexpensive desktop fluorescence reader ($6,000) instead of expensive fluorescence microscopes. By using the immuno-wall devices and the fluorescence reader, the high-sensitivity C-reactive protein assays were performed with sample-in-answer-out in 15 minutes.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages935-937
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Fluorescence
Antigens
Microchannels
Antibodies
Washing
Assays
Microscopes
Proteins

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Kasama, T., Hasegawa, Y., Kondo, H., Ozawa, T., Kaji, N., Tokeshi, M., & Baba, Y. (2014). Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 935-937). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. / Kasama, Toshihiro; Hasegawa, Yutaka; Kondo, Haruyasu; Ozawa, Tsutomu; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 935-937 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kasama, T, Hasegawa, Y, Kondo, H, Ozawa, T, Kaji, N, Tokeshi, M & Baba, Y 2014, Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 935-937, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Kasama T, Hasegawa Y, Kondo H, Ozawa T, Kaji N, Tokeshi M et al. Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 935-937. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
Kasama, Toshihiro ; Hasegawa, Yutaka ; Kondo, Haruyasu ; Ozawa, Tsutomu ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 935-937 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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