Abstract
We have developed a novel immunoassay device which has a long and thin wall structure inside a microchannel, hence the name 'immuno-wall device'. Unreacted antigens and antibodies were completely removed by just immersing the device in a washing buffer for 1 minute. The long structure also allowed us to analyze fluorescence intensity by using inexpensive desktop fluorescence reader ($6,000) instead of expensive fluorescence microscopes. By using the immuno-wall devices and the fluorescence reader, the high-sensitivity C-reactive protein assays were performed with sample-in-answer-out in 15 minutes.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 935-937 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - Jan 1 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: Oct 26 2014 → Oct 30 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country | United States |
| City | San Antonio |
| Period | 10/26/14 → 10/30/14 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay. / Kasama, Toshihiro; Hasegawa, Yutaka; Kondo, Haruyasu; Ozawa, Tsutomu; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 935-937 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Development of immuno-wall devices and a mobile fluorescence reader for on-site sample-to-answer immunoassay
AU - Kasama, Toshihiro
AU - Hasegawa, Yutaka
AU - Kondo, Haruyasu
AU - Ozawa, Tsutomu
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2014/1/1
Y1 - 2014/1/1
N2 - We have developed a novel immunoassay device which has a long and thin wall structure inside a microchannel, hence the name 'immuno-wall device'. Unreacted antigens and antibodies were completely removed by just immersing the device in a washing buffer for 1 minute. The long structure also allowed us to analyze fluorescence intensity by using inexpensive desktop fluorescence reader ($6,000) instead of expensive fluorescence microscopes. By using the immuno-wall devices and the fluorescence reader, the high-sensitivity C-reactive protein assays were performed with sample-in-answer-out in 15 minutes.
AB - We have developed a novel immunoassay device which has a long and thin wall structure inside a microchannel, hence the name 'immuno-wall device'. Unreacted antigens and antibodies were completely removed by just immersing the device in a washing buffer for 1 minute. The long structure also allowed us to analyze fluorescence intensity by using inexpensive desktop fluorescence reader ($6,000) instead of expensive fluorescence microscopes. By using the immuno-wall devices and the fluorescence reader, the high-sensitivity C-reactive protein assays were performed with sample-in-answer-out in 15 minutes.
UR - http://www.scopus.com/inward/record.url?scp=84941702094&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941702094&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941702094
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 935
EP - 937
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
ER -