Development of liver regenerative therapy using glycoside-modified bone marrow cells

Ryosuke Misawa, Hirohiko Ise, Masafumi Takahashi, Hajime Morimoto, Eiji Kobayashi, Shin Ichi Miyagawa, Uichi Ikeda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Several recent studies have reported that bone marrow cells (BMCs) have the ability to generate functional hepatocytes. However, the efficiency at which BMC transplantation generates functional hepatocytes is rather low. We assumed that if BMCs accumulated directly in liver, the functional BMC-derived hepatocytes should increase efficiently. We tried to increase the accumulation of BMCs directly in liver through the interaction between hepatic asialoglycoprotein receptor and desialylated BMCs. Desialylated BMCs were produced with treatment of neuraminidase. Desialylated BMCs that expressed green fluorescent protein (GFP) were injected into Long Evans Cinnamon (LEC) rats, a human Wilson's disease model, intravenously. At 3 and 5 months after transplantation, GFP-expressing hepatocyte nodules appeared in the liver of these BMC-transplanted LEC rats. These findings suggest that the functional BMC-derived hepatocytes can be generated by the direct accumulation of BMCs and that this strategy is new BMC therapy for liver regeneration.

Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number2
DOIs
Publication statusPublished - Apr 7 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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