Development of multidrug resistance type I Cmdr1 expression in chicken embryonic gonads

K. Yamada, N. Shibuya, S. Aramaki, S. Okuno, T. Soh, N. Yamauchi, M. A. Hattori

Research output: Contribution to journalArticle

Abstract

A primary role of P-glycoprotein (P-gp), encoded by the multidrug resistance type I gene, is to protect against naturally occurring xenotoxics. Recently, the preferential expression of chicken multidrug resistance type I (Cmdr1) was identified in the embryonic gonads during the early periods of development. Here we investigated the expression of Cmdr1 and P-gp in the gonads during embryogenesis, and compared to that in the ovarian follicles of domestic hens (Gallus gallus). As revealed by immunohistochemistry, P-gp was highly expressed in theca cells of mature follicles, whereas the expression was low in immature follicles. Immunohistochemical analysis showed that expression of Cmdr1-type P-gp was very low in embryonic gonads. Cmdr1 mRNA was undetectable in the gonads of 5-day embryos (E5) by RT-PCR, whereas Cmdr1 mRNA was significantly detectable in the developing gonads at E9 and E21. In the testicular tissues, germ cells were distributed along developing seminiferous cords as identified by a specific marker gene, whereas Cmdr1-type P-gp positive cells were observed evenly on testicular tissues. Collectively, it is concluded that Cmdr1 expression is initiated in the chicken ovary and testis after sexual differentiation, but expression of Cmdr1-type P-gp is very low through embryogenesis.

Original languageEnglish
Pages (from-to)928-933
Number of pages6
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume147
Issue number4
DOIs
Publication statusPublished - Aug 1 2007

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Gonads
Multiple Drug Resistance
P-Glycoprotein
Chickens
Embryonic Development
Genes
Cells
Tissue
Theca Cells
MDR Genes
Sex Differentiation
Messenger RNA
Ovarian Follicle
Germ Cells
Testis
Ovary
Embryonic Structures
Immunohistochemistry
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

Development of multidrug resistance type I Cmdr1 expression in chicken embryonic gonads. / Yamada, K.; Shibuya, N.; Aramaki, S.; Okuno, S.; Soh, T.; Yamauchi, N.; Hattori, M. A.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 147, No. 4, 01.08.2007, p. 928-933.

Research output: Contribution to journalArticle

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