TY - JOUR
T1 - Development of multidrug resistance type I P-glycoprotein function during in vitro maturation of porcine oocyte
AU - Arai, Manabu
AU - Yamauchi, Nobuhiko
AU - Fukuda, Hiroaki
AU - Soh, Tomoki
AU - Hattori, Masa Aki
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Sciences (JSPS; 16380200). We are grateful to Dr. St. Ruetz (Novartis Pharma AG, Basel, Switzerland) for the supply of PSC-833.
PY - 2006/1
Y1 - 2006/1
N2 - The present study was performed to evaluate the expression and function of P-glycoprotein (P-gp) encoded the multidrug resistance type I (MDR1) gene, protecting from xenotoxics, in porcine oocyte during in vitro maturation. Cumulus-oocyte complexes (COCs) were cultured to obtain the germinal vesicle (GV), first metaphase and second metaphase (MII) oocytes. The P-gp function was assessed by means of the rhodamine 6G (R6G) efflux from oocytes with P-gp inhibitors such as verapamil and PSC-833. The MDR1 transcript was detected in the GV and MII oocytes by RT-PCR analysis using primer sets based on the human gene. P-gp inhibitors significantly blocked the R6G efflux from the MII oocytes, whereas the reagents were ineffective in the GV oocytes. The R6G efflux from oocytes was accelerated at the MII stage more than at the GV stage. Thus, the MDR1-type P-gp function is poor at the GV stage, but the function improves during oocyte maturation.
AB - The present study was performed to evaluate the expression and function of P-glycoprotein (P-gp) encoded the multidrug resistance type I (MDR1) gene, protecting from xenotoxics, in porcine oocyte during in vitro maturation. Cumulus-oocyte complexes (COCs) were cultured to obtain the germinal vesicle (GV), first metaphase and second metaphase (MII) oocytes. The P-gp function was assessed by means of the rhodamine 6G (R6G) efflux from oocytes with P-gp inhibitors such as verapamil and PSC-833. The MDR1 transcript was detected in the GV and MII oocytes by RT-PCR analysis using primer sets based on the human gene. P-gp inhibitors significantly blocked the R6G efflux from the MII oocytes, whereas the reagents were ineffective in the GV oocytes. The R6G efflux from oocytes was accelerated at the MII stage more than at the GV stage. Thus, the MDR1-type P-gp function is poor at the GV stage, but the function improves during oocyte maturation.
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U2 - 10.1016/j.reprotox.2005.05.004
DO - 10.1016/j.reprotox.2005.05.004
M3 - Article
C2 - 15993035
AN - SCOPUS:30044435267
SN - 0890-6238
VL - 21
SP - 34
EP - 41
JO - Reproductigve Toxicoloy
JF - Reproductigve Toxicoloy
IS - 1
ER -