Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering

Azizah Intan Pangesty, Mitsugu Todo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cardiovascular disease remains to be the major cause of death in Indonesia by 2018. Treatment to replace the ruptured blood vessel by a bypass surgery is often limited by the availability of autologous vascular, while the use of synthetic conduit is challenged by the mechanical mismatch and foreign body reactions. Tissue engineering offers a new approach to create artificial blood vessel with growth potential similar to the native tissue. This study aimed to develop the multiple layers of the cylindrical scaffold made of poly (lactic acid-co-ϵ-caprolactone) (PLCL) by the phase separation and the freeze drying method. The three types of scaffolds were fabricated: single layer, double and triple layer scaffold. The effect to the microstructural behaviour was observed using FESEM. The mechanical properties were characterized using ring tensile test. The biological properties including cell attachment and proliferation using endothelial cells (ECs) were evaluated during one week of culture. SEM observation revealed that the pore size increased as the number of layer increased. Single, double and triple layers of scaffolds had the average pore size of 219 μm 2 , 744 μm 2 and 684 μm 2 , respectively. Meanwhile, the porosity significantly decreased with the increase number of layers. The porosity of single, double and triple layer scaffolds were 92.8%, 68.9 %, and 64.9%, respectively. As the porosity decreased, the mechanical properties including elastic modulus, tensile strength and burst pressure improved in significant amount. The elastic modulus increased 2 folds from the single layer (0.98 MPa), double layers (7.36 MPa) and triple layers (15.45 MPa) scaffolds. The tensile strength of single, double and triple layer scaffold were 216 kPa, 1039 kPa, and 1453 kPa, respectively. The burst pressure of the single layer scaffold was 119 mmHg and increased in double layers and triple layers scaffolds, 305 mmHg and 604 mmHg, respectively. An increased of cell proliferation on the PLCL scaffold during one week of culture indicated that the scaffold is biocompatible for tissue regeneration. This study demonstrated that the mechanical properties can be controlled by creating multiple layers of the cylindrical scaffold. The double and triple layers cylindrical scaffolds are potential candidates for vascular tissue engineering application.

Original languageEnglish
Title of host publicationProceedings of 2018 2nd International Conference on Biomedical Engineering
Subtitle of host publicationSmart Technology for Better Society, IBIOMED 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages138-142
Number of pages5
ISBN (Electronic)9781538647363
DOIs
Publication statusPublished - Nov 13 2018
Event2nd International Conference on Biomedical Engineering, IBIOMED 2018 - Kuta, Bali, Indonesia
Duration: Jul 24 2018Jul 26 2018

Publication series

NameProceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018

Other

Other2nd International Conference on Biomedical Engineering, IBIOMED 2018
CountryIndonesia
CityKuta, Bali
Period7/24/187/26/18

Fingerprint

tissue engineering
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Blood Vessels
Porosity
Tensile Strength
Elastic Modulus
Cell Proliferation
Foreign-Body Reaction
Blood Substitutes
Pressure
Indonesia
Freeze Drying
Regeneration
Cause of Death
porosity
Cardiovascular Diseases
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Biomedical Engineering
  • Health Informatics
  • Instrumentation

Cite this

Pangesty, A. I., & Todo, M. (2018). Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering. In Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018 (pp. 138-142). [8534874] (Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IBIOMED.2018.8534874

Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering. / Pangesty, Azizah Intan; Todo, Mitsugu.

Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 138-142 8534874 (Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pangesty, AI & Todo, M 2018, Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering. in Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018., 8534874, Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018, Institute of Electrical and Electronics Engineers Inc., pp. 138-142, 2nd International Conference on Biomedical Engineering, IBIOMED 2018, Kuta, Bali, Indonesia, 7/24/18. https://doi.org/10.1109/IBIOMED.2018.8534874
Pangesty AI, Todo M. Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering. In Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 138-142. 8534874. (Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018). https://doi.org/10.1109/IBIOMED.2018.8534874
Pangesty, Azizah Intan ; Todo, Mitsugu. / Development of Multiple Layers of PLCL Scaffold for Vascular Tissue Engineering. Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 138-142 (Proceedings of 2018 2nd International Conference on Biomedical Engineering: Smart Technology for Better Society, IBIOMED 2018).
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