Development of nanoparticles sizing method based on fluorescence polarization

Yuki Ishizaki, Terutake Hayashi, Masaki Michihata, Yasuhiro Takaya

Research output: Contribution to conferencePaper

Abstract

We suggest a novel nanoparticle sizing method based on fluorescence polarization analysis. Particle size evaluation can be achieved by measuring the rotational diffusion coefficient, which is sensitive to the particle size. We develop a rotational diffusion coefficient measurement system and a fluorescent probe, which is labelled to particle. In order to verify the feasibility of the proposed method, fundamental experiments are performed. We measure the rotational diffusion coefficients of gold nanoparticles, whose diameters are 5 nm, 10 nm and 15 nm, using the developed system. The measured rotational diffusion coefficients decrease with increasing the particle size. It indicates that nanoparticle size, which is below 15 nm, can be measured with at least 5 nm resolution.

Original languageEnglish
Pages319-322
Number of pages4
Publication statusPublished - 2013
Event11th IMEKO TC14 International Symposium on Measurement and Quality Control, ISMQC 2013 - Cracow and Kielce, Poland
Duration: Sep 11 2013Sep 13 2013

Other

Other11th IMEKO TC14 International Symposium on Measurement and Quality Control, ISMQC 2013
CountryPoland
CityCracow and Kielce
Period9/11/139/13/13

All Science Journal Classification (ASJC) codes

  • Safety, Risk, Reliability and Quality

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    Ishizaki, Y., Hayashi, T., Michihata, M., & Takaya, Y. (2013). Development of nanoparticles sizing method based on fluorescence polarization. 319-322. Paper presented at 11th IMEKO TC14 International Symposium on Measurement and Quality Control, ISMQC 2013, Cracow and Kielce, Poland.