Development of nanoparticles sizing method based on fluorescence polarization

Yuki Ishizaki, Terutake Hayashi, Masaki Michihata, Yasuhiro Takaya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We suggest a novel nanoparticle sizing method based on fluorescence polarization analysis. Particle size evaluation can be achieved by measuring the rotational diffusion coefficient, which is sensitive to the particle size. We develop a rotational diffusion coefficient measurement system and a fluorescent probe, which is labelled to particle. In order to verify the feasibility of the proposed method, fundamental experiments are performed. We measure the rotational diffusion coefficients of gold nanoparticles, whose diameters are 5 nm, 10 nm and 15 nm, using the developed system. The measured rotational diffusion coefficients decrease with increasing the particle size. It indicates that nanoparticle size, which is below 15 nm, can be measured with at least 5 nm resolution.

Original languageEnglish
Title of host publication11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014
PublisherIMEKO-International Measurement Federation Secretariat
Pages341-344
Number of pages4
ISBN (Print)9781632667311
Publication statusPublished - 2014
Event11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014 - Tsukuba, Japan
Duration: Sep 3 2014Sep 5 2014

Publication series

Name11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014

Other

Other11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014
CountryJapan
CityTsukuba
Period9/3/149/5/14

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)

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  • Cite this

    Ishizaki, Y., Hayashi, T., Michihata, M., & Takaya, Y. (2014). Development of nanoparticles sizing method based on fluorescence polarization. In 11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014 (pp. 341-344). (11th IMEKO TC14 Symposium on Laser Metrology for Precision Measurement and Inspection in Industry, LMPMI 2014). IMEKO-International Measurement Federation Secretariat.