Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stål.) in indica rice 'ADR52'

Asanori Yara, Cong Nguyen Phi, Masaya Matsumura, Atsushi Yoshimura, Hideshi Yasui

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The brown planthopper [BPH; Nilaparvata lugens (Stål.)] is one of the most destructive insect pests in Asian rice-growing areas. Two genes conferring resistance to BPH, BPH25(t) and BPH26(t) derived from a BPHresistant indica rice cultivar, Oryza sativa ADR52, have been identified. However, they are linked to genes conferring late heading and hybrid spikelet sterility. To eliminate these unfavorable traits (linkage drag), we generated BC6F1 populations carrying BPH25(t) or BPH26(t) in a BPH-susceptible japonica cultivar, Taichung 65, through marker-assisted selection. We selected three near-isogenic lines (NILs) carrying BPH25(t) without late heading date and one NIL carrying BPH26(t) without spikelet sterility from BC6F2 progeny that showed between 96.3 and 99.8% of the Taichung 65 genetic background through whole-genome survey. In antibiosis testing, the rates of surviving insects and of females with swollen abdomens were lower on the NILs than on Taichung 65, indicating that bph25(t) and Bph26(t) alleles from ADR52 controlled the resistance to BPH. The NILs will serve as useful resources for (1) monitoring BPH virulence and for (2) increasing resistance to BPH.

Original languageEnglish
Pages (from-to)639-647
Number of pages9
JournalBreeding Science
Volume60
Issue number5
DOIs
Publication statusPublished - Dec 1 2010

Fingerprint

Nilaparvata lugens
isogenic lines
rice
Infertility
Insects
heading
Antibiosis
inflorescences
Abdomen
Genes
antibiosis
Virulence
cultivars
Alleles
marker-assisted selection
Genome
abdomen
insect pests
genetic background
linkage (genetics)

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stål.) in indica rice 'ADR52'. / Yara, Asanori; Phi, Cong Nguyen; Matsumura, Masaya; Yoshimura, Atsushi; Yasui, Hideshi.

In: Breeding Science, Vol. 60, No. 5, 01.12.2010, p. 639-647.

Research output: Contribution to journalArticle

@article{9ad94cb801eb452fa3a437926b58b7be,
title = "Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (St{\aa}l.) in indica rice 'ADR52'",
abstract = "The brown planthopper [BPH; Nilaparvata lugens (St{\aa}l.)] is one of the most destructive insect pests in Asian rice-growing areas. Two genes conferring resistance to BPH, BPH25(t) and BPH26(t) derived from a BPHresistant indica rice cultivar, Oryza sativa ADR52, have been identified. However, they are linked to genes conferring late heading and hybrid spikelet sterility. To eliminate these unfavorable traits (linkage drag), we generated BC6F1 populations carrying BPH25(t) or BPH26(t) in a BPH-susceptible japonica cultivar, Taichung 65, through marker-assisted selection. We selected three near-isogenic lines (NILs) carrying BPH25(t) without late heading date and one NIL carrying BPH26(t) without spikelet sterility from BC6F2 progeny that showed between 96.3 and 99.8{\%} of the Taichung 65 genetic background through whole-genome survey. In antibiosis testing, the rates of surviving insects and of females with swollen abdomens were lower on the NILs than on Taichung 65, indicating that bph25(t) and Bph26(t) alleles from ADR52 controlled the resistance to BPH. The NILs will serve as useful resources for (1) monitoring BPH virulence and for (2) increasing resistance to BPH.",
author = "Asanori Yara and Phi, {Cong Nguyen} and Masaya Matsumura and Atsushi Yoshimura and Hideshi Yasui",
year = "2010",
month = "12",
day = "1",
doi = "10.1270/jsbbs.60.639",
language = "English",
volume = "60",
pages = "639--647",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "5",

}

TY - JOUR

T1 - Development of near-isogenic lines for BPH25(t) and BPH26(t), which confer resistance to the brown planthopper, Nilaparvata lugens (Stål.) in indica rice 'ADR52'

AU - Yara, Asanori

AU - Phi, Cong Nguyen

AU - Matsumura, Masaya

AU - Yoshimura, Atsushi

AU - Yasui, Hideshi

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The brown planthopper [BPH; Nilaparvata lugens (Stål.)] is one of the most destructive insect pests in Asian rice-growing areas. Two genes conferring resistance to BPH, BPH25(t) and BPH26(t) derived from a BPHresistant indica rice cultivar, Oryza sativa ADR52, have been identified. However, they are linked to genes conferring late heading and hybrid spikelet sterility. To eliminate these unfavorable traits (linkage drag), we generated BC6F1 populations carrying BPH25(t) or BPH26(t) in a BPH-susceptible japonica cultivar, Taichung 65, through marker-assisted selection. We selected three near-isogenic lines (NILs) carrying BPH25(t) without late heading date and one NIL carrying BPH26(t) without spikelet sterility from BC6F2 progeny that showed between 96.3 and 99.8% of the Taichung 65 genetic background through whole-genome survey. In antibiosis testing, the rates of surviving insects and of females with swollen abdomens were lower on the NILs than on Taichung 65, indicating that bph25(t) and Bph26(t) alleles from ADR52 controlled the resistance to BPH. The NILs will serve as useful resources for (1) monitoring BPH virulence and for (2) increasing resistance to BPH.

AB - The brown planthopper [BPH; Nilaparvata lugens (Stål.)] is one of the most destructive insect pests in Asian rice-growing areas. Two genes conferring resistance to BPH, BPH25(t) and BPH26(t) derived from a BPHresistant indica rice cultivar, Oryza sativa ADR52, have been identified. However, they are linked to genes conferring late heading and hybrid spikelet sterility. To eliminate these unfavorable traits (linkage drag), we generated BC6F1 populations carrying BPH25(t) or BPH26(t) in a BPH-susceptible japonica cultivar, Taichung 65, through marker-assisted selection. We selected three near-isogenic lines (NILs) carrying BPH25(t) without late heading date and one NIL carrying BPH26(t) without spikelet sterility from BC6F2 progeny that showed between 96.3 and 99.8% of the Taichung 65 genetic background through whole-genome survey. In antibiosis testing, the rates of surviving insects and of females with swollen abdomens were lower on the NILs than on Taichung 65, indicating that bph25(t) and Bph26(t) alleles from ADR52 controlled the resistance to BPH. The NILs will serve as useful resources for (1) monitoring BPH virulence and for (2) increasing resistance to BPH.

UR - http://www.scopus.com/inward/record.url?scp=78751477560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78751477560&partnerID=8YFLogxK

U2 - 10.1270/jsbbs.60.639

DO - 10.1270/jsbbs.60.639

M3 - Article

AN - SCOPUS:78751477560

VL - 60

SP - 639

EP - 647

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 5

ER -