Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane

Toshiaki Takezawa, Maya Fukuda, Winnette Mcintosh-Ambrose, Ji Ae Ko, Jennifer Elisseeff, Sanae Haga, Michitaka Ozaki, Kiyoko Kato, Pi Chao Wang, Tadashi Uchino, Teruo Nishida

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

The white of an egg, rendered opaque by boiling, can be converted into a thin, transparent and rigid material like glass by evaporating the moisture. This phenomenon is known as the vitrification of heat-denatured proteins.We applied vitrification technology to a collagen gel and converted it into a rigid glass-like material. We attempted to rehydrate the glass-like material and succeeded in preparing a novel stable state of collagen gel that was a thin and transparent membrane with excellent gel strength and protein permeability.We called it "collagen vitrigel"because it was produced from the vitrification process of a traditional hydrogel. Further, a framework-embedded collagen vitrigel membrane that can be easily turned inside out with tweezers was prepared by inserting a nylon membrane ring in the collagen sol prior to the gelation, thereby allowing the membrane to function as a removable cell culture substratum. Different types of anchorage-dependent cells could be cultured on both surfaces of the substratum by the manipulation of two-dimensional cultures, and consequently a three-dimensional crosstalk model with paracrine effects from each cell type was reconstructed. Also, the collagen vitrigel membrane containing a bioactive molecule provided a drug delivery system (DDS) with sustainable release. In this review, we summarize the recent progress of applied studies using the collagen vitrigel membrane as follows: a corneal model for eye irritant and permeability tests, a skin model for sensitization test, a renal glomerular model for evaluating blood filtration, an endometrial model for developing a new treatment and a DDS of hepatocyte growth factor for improving liver disorder.

Original languageEnglish
Pages (from-to)565-574
Number of pages10
JournalYakugaku Zasshi
Volume130
Issue number4
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

Collagen
Cell Culture Techniques
Membranes
Vitrification
Glass
Gels
Drug Delivery Systems
Permeability
Egg White
Hepatocyte Growth Factor
Irritants
Hydrogel
Nylons
Polymethyl Methacrylate
Skin Tests
Proteins
Hot Temperature
Technology
Kidney
Liver

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Takezawa, T., Fukuda, M., Mcintosh-Ambrose, W., Ko, J. A., Elisseeff, J., Haga, S., ... Nishida, T. (2010). Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane. Yakugaku Zasshi, 130(4), 565-574. https://doi.org/10.1248/yakushi.130.565

Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane. / Takezawa, Toshiaki; Fukuda, Maya; Mcintosh-Ambrose, Winnette; Ko, Ji Ae; Elisseeff, Jennifer; Haga, Sanae; Ozaki, Michitaka; Kato, Kiyoko; Wang, Pi Chao; Uchino, Tadashi; Nishida, Teruo.

In: Yakugaku Zasshi, Vol. 130, No. 4, 01.01.2010, p. 565-574.

Research output: Contribution to journalReview article

Takezawa, T, Fukuda, M, Mcintosh-Ambrose, W, Ko, JA, Elisseeff, J, Haga, S, Ozaki, M, Kato, K, Wang, PC, Uchino, T & Nishida, T 2010, 'Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane', Yakugaku Zasshi, vol. 130, no. 4, pp. 565-574. https://doi.org/10.1248/yakushi.130.565
Takezawa T, Fukuda M, Mcintosh-Ambrose W, Ko JA, Elisseeff J, Haga S et al. Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane. Yakugaku Zasshi. 2010 Jan 1;130(4):565-574. https://doi.org/10.1248/yakushi.130.565
Takezawa, Toshiaki ; Fukuda, Maya ; Mcintosh-Ambrose, Winnette ; Ko, Ji Ae ; Elisseeff, Jennifer ; Haga, Sanae ; Ozaki, Michitaka ; Kato, Kiyoko ; Wang, Pi Chao ; Uchino, Tadashi ; Nishida, Teruo. / Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane. In: Yakugaku Zasshi. 2010 ; Vol. 130, No. 4. pp. 565-574.
@article{3bcb4a98750b477da4344337306fb0c6,
title = "Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane",
abstract = "The white of an egg, rendered opaque by boiling, can be converted into a thin, transparent and rigid material like glass by evaporating the moisture. This phenomenon is known as the vitrification of heat-denatured proteins.We applied vitrification technology to a collagen gel and converted it into a rigid glass-like material. We attempted to rehydrate the glass-like material and succeeded in preparing a novel stable state of collagen gel that was a thin and transparent membrane with excellent gel strength and protein permeability.We called it {"}collagen vitrigel{"}because it was produced from the vitrification process of a traditional hydrogel. Further, a framework-embedded collagen vitrigel membrane that can be easily turned inside out with tweezers was prepared by inserting a nylon membrane ring in the collagen sol prior to the gelation, thereby allowing the membrane to function as a removable cell culture substratum. Different types of anchorage-dependent cells could be cultured on both surfaces of the substratum by the manipulation of two-dimensional cultures, and consequently a three-dimensional crosstalk model with paracrine effects from each cell type was reconstructed. Also, the collagen vitrigel membrane containing a bioactive molecule provided a drug delivery system (DDS) with sustainable release. In this review, we summarize the recent progress of applied studies using the collagen vitrigel membrane as follows: a corneal model for eye irritant and permeability tests, a skin model for sensitization test, a renal glomerular model for evaluating blood filtration, an endometrial model for developing a new treatment and a DDS of hepatocyte growth factor for improving liver disorder.",
author = "Toshiaki Takezawa and Maya Fukuda and Winnette Mcintosh-Ambrose and Ko, {Ji Ae} and Jennifer Elisseeff and Sanae Haga and Michitaka Ozaki and Kiyoko Kato and Wang, {Pi Chao} and Tadashi Uchino and Teruo Nishida",
year = "2010",
month = "1",
day = "1",
doi = "10.1248/yakushi.130.565",
language = "English",
volume = "130",
pages = "565--574",
journal = "Yakugaku Zasshi",
issn = "0031-6903",
publisher = "公益社団法人 日本薬学会",
number = "4",

}

TY - JOUR

T1 - Development of novel cell culture systems utilizing the advantages of collagen vitrigel membrane

AU - Takezawa, Toshiaki

AU - Fukuda, Maya

AU - Mcintosh-Ambrose, Winnette

AU - Ko, Ji Ae

AU - Elisseeff, Jennifer

AU - Haga, Sanae

AU - Ozaki, Michitaka

AU - Kato, Kiyoko

AU - Wang, Pi Chao

AU - Uchino, Tadashi

AU - Nishida, Teruo

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The white of an egg, rendered opaque by boiling, can be converted into a thin, transparent and rigid material like glass by evaporating the moisture. This phenomenon is known as the vitrification of heat-denatured proteins.We applied vitrification technology to a collagen gel and converted it into a rigid glass-like material. We attempted to rehydrate the glass-like material and succeeded in preparing a novel stable state of collagen gel that was a thin and transparent membrane with excellent gel strength and protein permeability.We called it "collagen vitrigel"because it was produced from the vitrification process of a traditional hydrogel. Further, a framework-embedded collagen vitrigel membrane that can be easily turned inside out with tweezers was prepared by inserting a nylon membrane ring in the collagen sol prior to the gelation, thereby allowing the membrane to function as a removable cell culture substratum. Different types of anchorage-dependent cells could be cultured on both surfaces of the substratum by the manipulation of two-dimensional cultures, and consequently a three-dimensional crosstalk model with paracrine effects from each cell type was reconstructed. Also, the collagen vitrigel membrane containing a bioactive molecule provided a drug delivery system (DDS) with sustainable release. In this review, we summarize the recent progress of applied studies using the collagen vitrigel membrane as follows: a corneal model for eye irritant and permeability tests, a skin model for sensitization test, a renal glomerular model for evaluating blood filtration, an endometrial model for developing a new treatment and a DDS of hepatocyte growth factor for improving liver disorder.

AB - The white of an egg, rendered opaque by boiling, can be converted into a thin, transparent and rigid material like glass by evaporating the moisture. This phenomenon is known as the vitrification of heat-denatured proteins.We applied vitrification technology to a collagen gel and converted it into a rigid glass-like material. We attempted to rehydrate the glass-like material and succeeded in preparing a novel stable state of collagen gel that was a thin and transparent membrane with excellent gel strength and protein permeability.We called it "collagen vitrigel"because it was produced from the vitrification process of a traditional hydrogel. Further, a framework-embedded collagen vitrigel membrane that can be easily turned inside out with tweezers was prepared by inserting a nylon membrane ring in the collagen sol prior to the gelation, thereby allowing the membrane to function as a removable cell culture substratum. Different types of anchorage-dependent cells could be cultured on both surfaces of the substratum by the manipulation of two-dimensional cultures, and consequently a three-dimensional crosstalk model with paracrine effects from each cell type was reconstructed. Also, the collagen vitrigel membrane containing a bioactive molecule provided a drug delivery system (DDS) with sustainable release. In this review, we summarize the recent progress of applied studies using the collagen vitrigel membrane as follows: a corneal model for eye irritant and permeability tests, a skin model for sensitization test, a renal glomerular model for evaluating blood filtration, an endometrial model for developing a new treatment and a DDS of hepatocyte growth factor for improving liver disorder.

UR - http://www.scopus.com/inward/record.url?scp=77950426628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950426628&partnerID=8YFLogxK

U2 - 10.1248/yakushi.130.565

DO - 10.1248/yakushi.130.565

M3 - Review article

C2 - 20372002

AN - SCOPUS:77950426628

VL - 130

SP - 565

EP - 574

JO - Yakugaku Zasshi

JF - Yakugaku Zasshi

SN - 0031-6903

IS - 4

ER -