Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

Akio Takahashi; Kazumasa Ogasawara; Naoto Matsuki; Koh Fujinaga; Takaaki Nakaya; Kazuyoshi Ikuta; Wattana Auwanit; Mitsuo Honda; Yoshinori Fukui; Takehiko Sasazuki; Kazuya Iwabuchi; Kazunori Onoé

doi:10.1016/S0264-410X(98)00042-5

Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

Akio Takahashi, Kazumasa Ogasawara, Naoto Matsuki, Koh Fujinaga, Takaaki Nakaya, Kazuyoshi Ikuta, Wattana Auwanit, Mitsuo Honda, Yoshinori Fukui, Takehiko Sasazuki, Kazuya Iwabuchi, Kazunori Onoé

Research output: Contribution to journal › Article › peer-review

Abstract

Peptide vaccines against HIV-1 were prepared according to the cassette theory that we had proposed previously. An amino acid sequence of B subtype consensus of the HIV-1 V3 region was introduced into the MHC binding component with a supermotif for various MHC class II. The peptide vaccines induced T-cell responses in the DQ6 mice in which only DQ6 molecules were expressed as MHC class II. By contrast, an original V3 peptide including the consensus sequence was non-immunogenic in the DQ6 mice. Antibodies obtained from the DQ6 mice immunized with the peptide vaccines neutralized laboratory B subtype strains of HIV-1 in vitro. It may be anticipated that these peptide vaccines protect infection of HIV-1 in DQ6 positive individuals.

Original language	English
Pages (from-to)	1537-1543
Number of pages	7
Journal	Vaccine
Volume	16
Issue number	16
DOIs	https://doi.org/10.1016/S0264-410X(98)00042-5
Publication status	Published - Oct 1998

All Science Journal Classification (ASJC) codes

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(98)00042-5

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title = "Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice",

abstract = "Peptide vaccines against HIV-1 were prepared according to the cassette theory that we had proposed previously. An amino acid sequence of B subtype consensus of the HIV-1 V3 region was introduced into the MHC binding component with a supermotif for various MHC class II. The peptide vaccines induced T-cell responses in the DQ6 mice in which only DQ6 molecules were expressed as MHC class II. By contrast, an original V3 peptide including the consensus sequence was non-immunogenic in the DQ6 mice. Antibodies obtained from the DQ6 mice immunized with the peptide vaccines neutralized laboratory B subtype strains of HIV-1 in vitro. It may be anticipated that these peptide vaccines protect infection of HIV-1 in DQ6 positive individuals.",

author = "Akio Takahashi and Kazumasa Ogasawara and Naoto Matsuki and Koh Fujinaga and Takaaki Nakaya and Kazuyoshi Ikuta and Wattana Auwanit and Mitsuo Honda and Yoshinori Fukui and Takehiko Sasazuki and Kazuya Iwabuchi and Kazunori Ono{\'e}",

note = "Funding Information: We are gratefult o Dr Diane Mathis for classI I KO mousea nd to Dr Michiyuki Kasai for biotinylateda nti-H-2A mAb (Y3P). This work is supportedi n part by Grant-in-Aid for ScientificR esearchb y the Ministry of Health and Welfare, and The Ministry of Education, Science,S ports, and Culture, Japan, Nishimura Aging Fund and TomakomaiE ast Hospital Grant.",

year = "1998",

month = oct,

doi = "10.1016/S0264-410X(98)00042-5",

language = "English",

volume = "16",

pages = "1537--1543",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

AU - Takahashi, Akio

AU - Ogasawara, Kazumasa

AU - Matsuki, Naoto

AU - Fujinaga, Koh

AU - Nakaya, Takaaki

AU - Ikuta, Kazuyoshi

AU - Auwanit, Wattana

AU - Honda, Mitsuo

AU - Fukui, Yoshinori

AU - Sasazuki, Takehiko

AU - Iwabuchi, Kazuya

AU - Onoé, Kazunori

N1 - Funding Information: We are gratefult o Dr Diane Mathis for classI I KO mousea nd to Dr Michiyuki Kasai for biotinylateda nti-H-2A mAb (Y3P). This work is supportedi n part by Grant-in-Aid for ScientificR esearchb y the Ministry of Health and Welfare, and The Ministry of Education, Science,S ports, and Culture, Japan, Nishimura Aging Fund and TomakomaiE ast Hospital Grant.

PY - 1998/10

Y1 - 1998/10

N2 - Peptide vaccines against HIV-1 were prepared according to the cassette theory that we had proposed previously. An amino acid sequence of B subtype consensus of the HIV-1 V3 region was introduced into the MHC binding component with a supermotif for various MHC class II. The peptide vaccines induced T-cell responses in the DQ6 mice in which only DQ6 molecules were expressed as MHC class II. By contrast, an original V3 peptide including the consensus sequence was non-immunogenic in the DQ6 mice. Antibodies obtained from the DQ6 mice immunized with the peptide vaccines neutralized laboratory B subtype strains of HIV-1 in vitro. It may be anticipated that these peptide vaccines protect infection of HIV-1 in DQ6 positive individuals.

AB - Peptide vaccines against HIV-1 were prepared according to the cassette theory that we had proposed previously. An amino acid sequence of B subtype consensus of the HIV-1 V3 region was introduced into the MHC binding component with a supermotif for various MHC class II. The peptide vaccines induced T-cell responses in the DQ6 mice in which only DQ6 molecules were expressed as MHC class II. By contrast, an original V3 peptide including the consensus sequence was non-immunogenic in the DQ6 mice. Antibodies obtained from the DQ6 mice immunized with the peptide vaccines neutralized laboratory B subtype strains of HIV-1 in vitro. It may be anticipated that these peptide vaccines protect infection of HIV-1 in DQ6 positive individuals.

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Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice

Abstract

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