Development of protein-labeling probes with a redesigned fluorogenic switch based on intramolecular association for no-wash live-cell imaging

Yuichiro Hori; Kyohei Nakaki; Motoki Sato; Shin Mizukami; Kazuya Kikuchi

doi:10.1002/anie.201200867

Development of protein-labeling probes with a redesigned fluorogenic switch based on intramolecular association for no-wash live-cell imaging

Yuichiro Hori, Kyohei Nakaki, Motoki Sato, Shin Mizukami, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Turn on the switch: Fluorogenic probes for protein labeling based on the photoactive yellow protein (PYP) tag were developed. The fluorescence of the probes is turned off by intramolecular association and switched on by the reversal of this interaction upon reaction with the PYP tag that is fused to the protein of interest (POI, see scheme). The rapid and specific labeling reaction enabled the imaging of cell-surface proteins without washing.

Original language	English
Pages (from-to)	5611-5614
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	23
DOIs	https://doi.org/10.1002/anie.201200867
Publication status	Published - Jun 4 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
Chemistry(all)

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10.1002/anie.201200867

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abstract = "Turn on the switch: Fluorogenic probes for protein labeling based on the photoactive yellow protein (PYP) tag were developed. The fluorescence of the probes is turned off by intramolecular association and switched on by the reversal of this interaction upon reaction with the PYP tag that is fused to the protein of interest (POI, see scheme). The rapid and specific labeling reaction enabled the imaging of cell-surface proteins without washing.",

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AU - Hori, Yuichiro

AU - Nakaki, Kyohei

AU - Sato, Motoki

AU - Mizukami, Shin

AU - Kikuchi, Kazuya

PY - 2012/6/4

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Development of protein-labeling probes with a redesigned fluorogenic switch based on intramolecular association for no-wash live-cell imaging

Abstract

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