Development of radioimmunoassay for human leptin

Kiminori Hosoda, Hiroaki Masuzaki, Yoshihiro Ogawa, Takashi Miyawaki, Junko Hiraoka, Ikuko Hanaoka, Akiko Yasuno, Toshiyuki Nomura, Yukio Fujisawa, Yasunao Yoshimasa, Shigeo Nishi, Yukio Yamori, Kazuwa Nakao

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Abstract

Using recombinant human leptin, we have produced an antiserum for human leptin and developed a radioimmunoassay (RIA) specific and sensitive for human leptin. We detected leptin-like immunoreactivity (-LI) in culture media of adipose tissue from subcutaneous abdominal fat in human. The plasma human leptin-LI concentration in nonobese subjects (17.6 < body mass index (BMI) < 23.0) was 5.6 ± 1.3 (mean ± SE) ng/ml, while that in obese subjects (29.0 < BMI) was 43.0 ± 43.0 ± 9.4 (mean ± SE) ng/ml. In gel permeation chromatographic analyses, leptin-LI in culture media and plasma consisted of single component emerging at the elution position of recombinant human leptin. These findings indicate that leptin is secreted from the adipose tissue into the circulating blood as a large molecular form corresponding to recombinant leptin. The RIA developed in the present study will be a powerful tool to investigate the physiological and pathophysiological significance of leptin in human.

Original languageEnglish
Pages (from-to)234-239
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number2
DOIs
Publication statusPublished - Apr 16 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hosoda, K., Masuzaki, H., Ogawa, Y., Miyawaki, T., Hiraoka, J., Hanaoka, I., Yasuno, A., Nomura, T., Fujisawa, Y., Yoshimasa, Y., Nishi, S., Yamori, Y., & Nakao, K. (1996). Development of radioimmunoassay for human leptin. Biochemical and Biophysical Research Communications, 221(2), 234-239. https://doi.org/10.1006/bbrc.1996.0579