Development of signaling echo method for cell-based quantitative efficacy evaluation of anti-cancer drugs in apoptosis without drug presence using high-precision surface plasmon resonance sensing

Hiroshi Nishijima, Atsushi Kosaihira, Junko Shibata, Toshihiro Ona

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We propose a rapid and label-free quantitative evaluation of anti-cancer drug efficacy in apoptosis using live cancer cells cultured on a sensor chip. The resultant custom-made, high-precision surface plasmon resonance (SPR) sensor monitors the inner mitochondrial membrane's potential change (ΔΨ m ). For trans-membrane anti-cancer drugs to be effective, continuous potential changes arising from the crossing of the membrane by the drug of interest prevent ΔΨ m monitoring. Herein, we report on a novel signaling echo method that avoids this disturbance; the cancer cells are incubated with a specific anti-cancer drug, with subsequent removal of the drug before SPR measurements. The cell reaction without any drug was monitored as the differential SPR angle rate of change for 10 min from 30 min after a sensor chip was set on a prism. The cell reaction after 60 min pre-incubation with a drug was significantly related to the conventional cell viability after 48 h (P <0.001). 2010

Original languageEnglish
Pages (from-to)529-534
Number of pages6
Journalanalytical sciences
Volume26
Issue number5
DOIs
Publication statusPublished - May 2010

Fingerprint

Surface plasmon resonance
Apoptosis
Pharmaceutical Preparations
Cells
Membranes
Sensors
Prisms
Labels
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

@article{c04aafb00bf24b1aaec778c1b202924d,
title = "Development of signaling echo method for cell-based quantitative efficacy evaluation of anti-cancer drugs in apoptosis without drug presence using high-precision surface plasmon resonance sensing",
abstract = "We propose a rapid and label-free quantitative evaluation of anti-cancer drug efficacy in apoptosis using live cancer cells cultured on a sensor chip. The resultant custom-made, high-precision surface plasmon resonance (SPR) sensor monitors the inner mitochondrial membrane's potential change (ΔΨ m ). For trans-membrane anti-cancer drugs to be effective, continuous potential changes arising from the crossing of the membrane by the drug of interest prevent ΔΨ m monitoring. Herein, we report on a novel signaling echo method that avoids this disturbance; the cancer cells are incubated with a specific anti-cancer drug, with subsequent removal of the drug before SPR measurements. The cell reaction without any drug was monitored as the differential SPR angle rate of change for 10 min from 30 min after a sensor chip was set on a prism. The cell reaction after 60 min pre-incubation with a drug was significantly related to the conventional cell viability after 48 h (P <0.001). 2010",
author = "Hiroshi Nishijima and Atsushi Kosaihira and Junko Shibata and Toshihiro Ona",
year = "2010",
month = "5",
doi = "10.2116/analsci.26.529",
language = "English",
volume = "26",
pages = "529--534",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "The Japan Society for Analytical Chemistry",
number = "5",

}

TY - JOUR

T1 - Development of signaling echo method for cell-based quantitative efficacy evaluation of anti-cancer drugs in apoptosis without drug presence using high-precision surface plasmon resonance sensing

AU - Nishijima, Hiroshi

AU - Kosaihira, Atsushi

AU - Shibata, Junko

AU - Ona, Toshihiro

PY - 2010/5

Y1 - 2010/5

N2 - We propose a rapid and label-free quantitative evaluation of anti-cancer drug efficacy in apoptosis using live cancer cells cultured on a sensor chip. The resultant custom-made, high-precision surface plasmon resonance (SPR) sensor monitors the inner mitochondrial membrane's potential change (ΔΨ m ). For trans-membrane anti-cancer drugs to be effective, continuous potential changes arising from the crossing of the membrane by the drug of interest prevent ΔΨ m monitoring. Herein, we report on a novel signaling echo method that avoids this disturbance; the cancer cells are incubated with a specific anti-cancer drug, with subsequent removal of the drug before SPR measurements. The cell reaction without any drug was monitored as the differential SPR angle rate of change for 10 min from 30 min after a sensor chip was set on a prism. The cell reaction after 60 min pre-incubation with a drug was significantly related to the conventional cell viability after 48 h (P <0.001). 2010

AB - We propose a rapid and label-free quantitative evaluation of anti-cancer drug efficacy in apoptosis using live cancer cells cultured on a sensor chip. The resultant custom-made, high-precision surface plasmon resonance (SPR) sensor monitors the inner mitochondrial membrane's potential change (ΔΨ m ). For trans-membrane anti-cancer drugs to be effective, continuous potential changes arising from the crossing of the membrane by the drug of interest prevent ΔΨ m monitoring. Herein, we report on a novel signaling echo method that avoids this disturbance; the cancer cells are incubated with a specific anti-cancer drug, with subsequent removal of the drug before SPR measurements. The cell reaction without any drug was monitored as the differential SPR angle rate of change for 10 min from 30 min after a sensor chip was set on a prism. The cell reaction after 60 min pre-incubation with a drug was significantly related to the conventional cell viability after 48 h (P <0.001). 2010

UR - http://www.scopus.com/inward/record.url?scp=77955805602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955805602&partnerID=8YFLogxK

U2 - 10.2116/analsci.26.529

DO - 10.2116/analsci.26.529

M3 - Article

C2 - 20467125

AN - SCOPUS:77955805602

VL - 26

SP - 529

EP - 534

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 5

ER -