Development of simple DNA markers for selecting trees with the male-sterile gene of cryptomeriajaponica "Sosyun"

Miyoko Tsubomura, Nomado Goda, Tomonori Hirao, Kentaro Mishima, Ken Ichi Konagaya, Miho Tamura, Makoto Takahashi, Atsushi Watanabe

Research output: Contribution to journalArticle

Abstract

We report the development of two types of simple DNA markers that can be used to easily identify genotypes of both the male-sterile and wild-type individuals at a locus, which is tightly linked to the causative gene of male sterility in the “Sosyun” clone of Cryptomeria japonica. Both the allele-specific primer (ASP) PCR marker and cleaved amplified polymorphic sequence (CAPS) marker were designed using previously reported single nucleotide polymorphism (SNP) information. The cDNA and genomic DNA of the EST reCj 19250, which is linked to the ms-1 locus, were sequenced for both the “Sosyun” and wild-type plus-tree “Usui 2” clones. Primers were designed for detecting both the sterile and wild-type SNPs using the genomic DNA sequences. For the ASP-PCR marker, homozygous recessive male-sterile individuals yielded a single 157-bp fragment, whereas the homozygous wild-type individuals yielded a single 345-bp fragment, and heterozygotes yielded both fragments. Similarly, for the CAPS marker, male-sterile individuals yielded a single 101-bp fragment after digestion, whereas wild-type ones yielded an intact single 125 -bp fragment, and heterozygotes yielded fragments of both sizes. These markers could be utilized for identifying male-sterile individuals during seedling production and for identifying heterozygous trees for future tree breeding.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume101
Issue number4
DOIs
Publication statusPublished - Jan 1 2019

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DNA
genetic markers
gene
heterozygosity
genes
clones
alleles
plus trees
genomics
tree breeding
loci
seedling production
Cryptomeria japonica
clone
male fertility
allele
single nucleotide polymorphism
sterility
digestion
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Forestry

Cite this

Development of simple DNA markers for selecting trees with the male-sterile gene of cryptomeriajaponica "Sosyun". / Tsubomura, Miyoko; Goda, Nomado; Hirao, Tomonori; Mishima, Kentaro; Konagaya, Ken Ichi; Tamura, Miho; Takahashi, Makoto; Watanabe, Atsushi.

In: Nihon Ringakkai Shi/Journal of the Japanese Forestry Society, Vol. 101, No. 4, 01.01.2019, p. 155-162.

Research output: Contribution to journalArticle

Tsubomura, Miyoko ; Goda, Nomado ; Hirao, Tomonori ; Mishima, Kentaro ; Konagaya, Ken Ichi ; Tamura, Miho ; Takahashi, Makoto ; Watanabe, Atsushi. / Development of simple DNA markers for selecting trees with the male-sterile gene of cryptomeriajaponica "Sosyun". In: Nihon Ringakkai Shi/Journal of the Japanese Forestry Society. 2019 ; Vol. 101, No. 4. pp. 155-162.
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