TY - JOUR
T1 - Development of simplified vasoactive intestinal peptide analogs with receptor selectivity and stability for human vasoactive intestinal peptide/pituitary adenylate cyclase-activating polypeptide receptors
AU - Igarashi, Hisato
AU - Ito, Tetsuhide
AU - Mantey, Samuel A.
AU - Pradhan, Tapas K.
AU - Hou, Wei
AU - Coy, David H.
AU - Jensen, Robert T.
PY - 2005/10
Y1 - 2005/10
N2 - Vasoactive intestinal peptide (VIP) is a widespread neurotransmitter whose physiological and pathophysiological actions are mediated by two receptor classes, VIP/pituitary adenylate cyclase-activating polypeptide (VPAC) 1 and VPAC2. VIP is 28-amino acid peptide that is rapidly degraded and simplified; metabolically stable analogs are needed. In this study, we use information from studies of the VIP pharmacophore for VPAC1/ VPAC2to design nine simplified VIP analogs that could have high affinity and selectivity for each VPAC or that retained high affinity for both VPACs and were metabolically stable. From binding studies of their abilities to directly interact with hVPAC (T47D cells, hVPAC1-transfected cells) and hVPAC2 (Sup T1-and VPAC2-transfected cells) and to stimulate adenylate cyclase in each, two analogs [(Ala 2,8,9,11,19,22,24,25,27,28)VIP and (Ala2,8,9,11,19,24-28) VIP] were found to have >2000-and >600-fold selectivity for hVPAC 1. None of the nine analogs had hVPAC2 selectivity. However, two simplified analogs [(Ala2,8,9, 16,19,24)VIP and (Ala2,8,9,16,19,24,25)VIP] retained high affinity and potency for both hVPACs. 125I-[Ala2,8,9,16,19,24,25]VIP was much more metabolically stable than 125I-VIP. The availability of these simplified analogs of VIP, which are metabolically stable and have either hVPAC1 selectivity or retain high affinity for both hVPACs, should be useful for exploring the role of VPAC subtypes in mediating VIPs' actions as well as being useful therapeutically and for exploring the usefulness of VIP receptor imaging of tumors and VIP receptor-mediated tumor cytotoxicity.
AB - Vasoactive intestinal peptide (VIP) is a widespread neurotransmitter whose physiological and pathophysiological actions are mediated by two receptor classes, VIP/pituitary adenylate cyclase-activating polypeptide (VPAC) 1 and VPAC2. VIP is 28-amino acid peptide that is rapidly degraded and simplified; metabolically stable analogs are needed. In this study, we use information from studies of the VIP pharmacophore for VPAC1/ VPAC2to design nine simplified VIP analogs that could have high affinity and selectivity for each VPAC or that retained high affinity for both VPACs and were metabolically stable. From binding studies of their abilities to directly interact with hVPAC (T47D cells, hVPAC1-transfected cells) and hVPAC2 (Sup T1-and VPAC2-transfected cells) and to stimulate adenylate cyclase in each, two analogs [(Ala 2,8,9,11,19,22,24,25,27,28)VIP and (Ala2,8,9,11,19,24-28) VIP] were found to have >2000-and >600-fold selectivity for hVPAC 1. None of the nine analogs had hVPAC2 selectivity. However, two simplified analogs [(Ala2,8,9, 16,19,24)VIP and (Ala2,8,9,16,19,24,25)VIP] retained high affinity and potency for both hVPACs. 125I-[Ala2,8,9,16,19,24,25]VIP was much more metabolically stable than 125I-VIP. The availability of these simplified analogs of VIP, which are metabolically stable and have either hVPAC1 selectivity or retain high affinity for both hVPACs, should be useful for exploring the role of VPAC subtypes in mediating VIPs' actions as well as being useful therapeutically and for exploring the usefulness of VIP receptor imaging of tumors and VIP receptor-mediated tumor cytotoxicity.
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U2 - 10.1124/jpet.105.088823
DO - 10.1124/jpet.105.088823
M3 - Article
C2 - 15994369
AN - SCOPUS:25644444563
SN - 0022-3565
VL - 315
SP - 370
EP - 381
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -