Development of specific adsorbent for cryogel

M. Ohmae, T. Nakamura, K. Miyamoto, Masayuki Tokita, T. Komai, M. Yonekawa, A. Kawamura, E. Sakashita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cryo precipitable proteins constituting in cryogel existed in patient's plasma with autoimmune diseases such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Cryofiltration was developed by Nose et al that plasma separated from the whole blood by extracorporeal blood circulation system was cooled and then cryogel is removed by the second filter. Therapeutic efficiency on these autoimmune diseases was reported. On the other hand, we have found that main components of cryogel were fibrinogen (Fbg) and fibronectin (FN). FN has molecular diversity of plasma FN (pFN) and cellular (cFN), so we investigated FN involved in cryogel. The result showed that EDA(+)FN, usually in extracellular matrix, is existed in high percentage. In this study, we prepared heparinoid cellulose sulfate as the specific adsorbent for cryogel and then examined its adsorption ability by using human plasma at 30°C. The result was that about 90% of EDA(+)FN was removed while only 50% of total FN (pFN+cFN), 20% of Fbg and ~13% of others were. Consequently this adsorbent seemed to be useful for the material of cryofiltration system.

Original languageEnglish
Pages (from-to)660-664
Number of pages5
JournalJapanese Journal of Artificial Organs
Volume23
Issue number3
Publication statusPublished - Jan 1 1994

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Cryogels
Fibronectins
Fibrinogen
Autoimmune Diseases
Heparinoids
Extracorporeal Circulation
Blood Circulation
Systemic Lupus Erythematosus
Adsorption
Extracellular Matrix
Rheumatoid Arthritis

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Ohmae, M., Nakamura, T., Miyamoto, K., Tokita, M., Komai, T., Yonekawa, M., ... Sakashita, E. (1994). Development of specific adsorbent for cryogel. Japanese Journal of Artificial Organs, 23(3), 660-664.

Development of specific adsorbent for cryogel. / Ohmae, M.; Nakamura, T.; Miyamoto, K.; Tokita, Masayuki; Komai, T.; Yonekawa, M.; Kawamura, A.; Sakashita, E.

In: Japanese Journal of Artificial Organs, Vol. 23, No. 3, 01.01.1994, p. 660-664.

Research output: Contribution to journalArticle

Ohmae, M, Nakamura, T, Miyamoto, K, Tokita, M, Komai, T, Yonekawa, M, Kawamura, A & Sakashita, E 1994, 'Development of specific adsorbent for cryogel', Japanese Journal of Artificial Organs, vol. 23, no. 3, pp. 660-664.
Ohmae M, Nakamura T, Miyamoto K, Tokita M, Komai T, Yonekawa M et al. Development of specific adsorbent for cryogel. Japanese Journal of Artificial Organs. 1994 Jan 1;23(3):660-664.
Ohmae, M. ; Nakamura, T. ; Miyamoto, K. ; Tokita, Masayuki ; Komai, T. ; Yonekawa, M. ; Kawamura, A. ; Sakashita, E. / Development of specific adsorbent for cryogel. In: Japanese Journal of Artificial Organs. 1994 ; Vol. 23, No. 3. pp. 660-664.
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