Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology

Hisao Matsuno, Jun Sekine, Hirofumi Yajima, Takeshi Seizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Structurally well-defined synthetic polymers with stereoregularity, chirality, helicity, and assembly are versatile materials for conjugation with biomolecules, and hybridization of these polymers with various biomolecules such as polypeptide and protein will expand its potentialities for biomedical applications. In this paper, peptides that bind to poly(L-lactide) (PLLA) were selected from the 7-mer peptide library. The spin-casting or casting film of PLLA was formed on a glass plate, and the biopanning was carried out on the PLLA film with the phage display peptide library. After several rounds of biopanning, sixteen individual sequences were obtained from the 30 phage clones. We quantified the binding property between the selected peptides and PLLA by the enzyme linked immunosorbent assay (ELISA).

Original languageEnglish
Title of host publication55th SPSJ Symposium on Macromolecules
Pages5410-5411
Number of pages2
Volume55
Edition2
Publication statusPublished - 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period9/20/069/22/06

Fingerprint

Peptides
Screening
Bacteriophages
Biomolecules
Casting
Chirality
Polypeptides
Polymers
Assays
Enzymes
Display devices
Proteins
Glass

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Matsuno, H., Sekine, J., Yajima, H., & Seizawa, T. (2006). Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology. In 55th SPSJ Symposium on Macromolecules (2 ed., Vol. 55, pp. 5410-5411)

Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology. / Matsuno, Hisao; Sekine, Jun; Yajima, Hirofumi; Seizawa, Takeshi.

55th SPSJ Symposium on Macromolecules. Vol. 55 2. ed. 2006. p. 5410-5411.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matsuno, H, Sekine, J, Yajima, H & Seizawa, T 2006, Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology. in 55th SPSJ Symposium on Macromolecules. 2 edn, vol. 55, pp. 5410-5411, 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan, 9/20/06.
Matsuno H, Sekine J, Yajima H, Seizawa T. Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology. In 55th SPSJ Symposium on Macromolecules. 2 ed. Vol. 55. 2006. p. 5410-5411
Matsuno, Hisao ; Sekine, Jun ; Yajima, Hirofumi ; Seizawa, Takeshi. / Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology. 55th SPSJ Symposium on Macromolecules. Vol. 55 2. ed. 2006. pp. 5410-5411
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