Development of synthetic poly(lactide)-binding peptides using combinatorial screening technology

Hisao Matsuno, Jun Sekine, Hirofumi Yajima, Takeshi Seizawa

Research output: Contribution to conferencePaperpeer-review

Abstract

Structurally well-defined synthetic polymers with stereoregularity, chirality, helicity, and assembly are versatile materials for conjugation with biomolecules, and hybridization of these polymers with various biomolecules such as polypeptide and protein will expand its potentialities for biomedical applications. In this paper, peptides that bind to poly(L-lactide) (PLLA) were selected from the 7-mer peptide library. The spin-casting or casting film of PLLA was formed on a glass plate, and the biopanning was carried out on the PLLA film with the phage display peptide library. After several rounds of biopanning, sixteen individual sequences were obtained from the 30 phage clones. We quantified the binding property between the selected peptides and PLLA by the enzyme linked immunosorbent assay (ELISA).

Original languageEnglish
Pages5410-5411
Number of pages2
Publication statusPublished - Dec 1 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period9/20/069/22/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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