Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon

D. V. Thao, M. Yamashita, A. Watanabe, S. Shiraishi

Research output: Contribution to journalArticle

Abstract

Pinus kesiya Royle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0. 198 to 0. 954, with an average of 0. 685. These polymorphic markers will serve as useful tools for studies of conservation genetics in P. kesiya and probably in its closely related species.

Original languageEnglish
Pages (from-to)405-407
Number of pages3
JournalConservation Genetics Resources
Volume5
Issue number2
DOIs
Publication statusPublished - May 3 2013

Fingerprint

Pinus kesiya
Pinus
Vietnam
Microsatellite Repeats
Alleles
microsatellite repeats
Polymerase Chain Reaction
Population
conservation genetics
loci
germplasm conservation
heterozygosity
polymerase chain reaction
allele
alleles
marker
methodology

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon. / Thao, D. V.; Yamashita, M.; Watanabe, A.; Shiraishi, S.

In: Conservation Genetics Resources, Vol. 5, No. 2, 03.05.2013, p. 405-407.

Research output: Contribution to journalArticle

Thao, D. V. ; Yamashita, M. ; Watanabe, A. ; Shiraishi, S. / Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon. In: Conservation Genetics Resources. 2013 ; Vol. 5, No. 2. pp. 405-407.
@article{83dc1c54241f444490e38bfece74ad3b,
title = "Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon",
abstract = "Pinus kesiya Royle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0. 198 to 0. 954, with an average of 0. 685. These polymorphic markers will serve as useful tools for studies of conservation genetics in P. kesiya and probably in its closely related species.",
author = "Thao, {D. V.} and M. Yamashita and A. Watanabe and S. Shiraishi",
year = "2013",
month = "5",
day = "3",
doi = "10.1007/s12686-012-9814-3",
language = "English",
volume = "5",
pages = "405--407",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon

AU - Thao, D. V.

AU - Yamashita, M.

AU - Watanabe, A.

AU - Shiraishi, S.

PY - 2013/5/3

Y1 - 2013/5/3

N2 - Pinus kesiya Royle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0. 198 to 0. 954, with an average of 0. 685. These polymorphic markers will serve as useful tools for studies of conservation genetics in P. kesiya and probably in its closely related species.

AB - Pinus kesiya Royle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0. 198 to 0. 954, with an average of 0. 685. These polymorphic markers will serve as useful tools for studies of conservation genetics in P. kesiya and probably in its closely related species.

UR - http://www.scopus.com/inward/record.url?scp=84876875316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876875316&partnerID=8YFLogxK

U2 - 10.1007/s12686-012-9814-3

DO - 10.1007/s12686-012-9814-3

M3 - Article

AN - SCOPUS:84876875316

VL - 5

SP - 405

EP - 407

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -