糖負荷試験の検体を利用した胃粘膜検査法の開発

Translated title of the contribution: Development of the Enzymatic Method for Maltose Permeability Test of Gastric Mucosa using the Oral Glucose Tolerance Samples

Susumu Osawa, Ayaka Ishibashi, Eisaku Hokazono, Yuzo Kayamori

Research output: Contribution to journalArticle

Abstract

The examination of gastric mucosa is performed both test gastrointestinal contrasting with X rays and pepsinogen test in serum. However, the being bombed with X-rays and an autoanalysis apparatus for exclusive immunoassay are necessary to take these tests. We developed instead of maltose permeability test of gastric mucosa using the oral glucose tolerance samples. Enzymatic method for oligosaccharide is changed from endogenous glucose to glucose-6-phosphate by hexokinase and was eliminated. The oligosaccharide which was absorbed from a gastric mucosa is determined of the α-glucosidase-glucoseoxidase-peroxidase detection system as the determination of equivalent to oligosaccharide concentration. This method could discover a gastric mucosa disorder easily.
Translated title of the contributionDevelopment of the Enzymatic Method for Maltose Permeability Test of Gastric Mucosa using the Oral Glucose Tolerance Samples
Original languageJapanese
Pages (from-to)270-277
JournalJapanese Journal of Clinical Laboratory Automation
Volume38
Issue number3
Publication statusPublished - Mar 2013

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