Development of the selective adsorbent for EDA containing fibronectin using heparin immobilized cellulose

Keiichi Miyashita, Eiji Sakashita, Keiichi Miyamoto, Masayuki Tokita, Takashi Komai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have noticed that extra domain A containing fibronectin (EDA(+)FN) is closely related to inflammatory diseases and accordingly investigated a selective adsorbent to remove EDA(+)FN, which may increase the severity of the symptoms, from the plasma of rheumatoid arthritis (RA) patients. Three types of heparin immobilized cellulose (HC) were prepared and their adsorptive properties were evaluated by batchwise adsorption tests. The results showed that EDA(+)FN was adsorbed more efficiently than total FN on HC. In particular HC in which heparin was immobilized to amino residue had an excellent capacity. The adsorption rates of EDA(+)FN, total FN and antithrombin-III were 89, 15 and 17% respectively. This adsorbent is extremely useful in selectively removing EDA(+)FN from plasma.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 1 1998

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

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