Development of triplex forming oligonucleotide including artificial nucleoside analogues for the antigene strategy

Yosuke Taniguchi, Shigeki Sasaki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The sequence-specific triplex formation against duplex DNA offers a potential basis for genome targeting technology, such as diagnostics, regulation of gene expression and sequencing technologies. In an antiparallel triplex DNA, a purine-rich triplex forming oligonucleotide (TFO) consisting of a dG, dA or T forms two reverse Hoogsteen hydrogen bonds with a GC, AT or AT base pair of the duplex DNA, respectively, with a high selectivity in a sequence specific manner. However, there is no natural nucleoside which can recognize the inverted CG and TA base pair of the duplex DNA. Therefore, the development of recognition molecules for the CG and TA inversion sites with a high stability and selectivity has been demanded for the triplex forming technology. In this chapter, we describe the design and synthesis of W-shaped nucleoside analogues (WNA-βT) and pseudo-dC derivatives (MeAP-ΨdC) for selective recognition of the TA and CG base pair, respectively, to expand the triplex-forming sequence.

Original languageEnglish
Title of host publicationSynthesis of Therapeutic Oligonucleotides
PublisherSpringer Singapore
Pages253-269
Number of pages17
ISBN (Electronic)9789811319129
ISBN (Print)9789811319112
DOIs
Publication statusPublished - Dec 8 2018

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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