Development of ultraviolet-excitation photothermal microscope and observation of a single nonstained biological cell

Noriyuki Fujii, Akira Harata

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    Abstract

    A system of ultraviolet-laser excitation microscopic photothermal lens imaging for observing chemical compounds in biological cells has been developed, in which a reflection objective lens is used for focusing an excitation laser beam at 260 nm and a probe laser beam at 780 nm. The system enables direct comparison of photothermal and transmitted-light-intensity images to an image of the same object obtained with a complementary metal-oxide-semiconductor image sensor (CMOS). A photothermal lens image of a single yeast cell was successfully obtained and the position of the photothermal images well corresponded to that of the CMOS images.

    Original languageEnglish
    Article number07GC09
    JournalJapanese journal of applied physics
    Volume48
    Issue number7 PART 2
    DOIs
    Publication statusPublished - Jul 2009

    All Science Journal Classification (ASJC) codes

    • Engineering(all)
    • Physics and Astronomy(all)

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