Development of vascular smooth muscle contractility by endothelium-derived transforming growth factor β proteins

Chiwaka Kimura, Shuhei Konishi, Maki Hasegawa, Masahiro Oike

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is well established that the release of vasodilators and vasoconstrictors from vascular endothelium regulates vascular smooth muscle contraction. In this report, we investigate the role of the endothelium in the development and maintenance of constitutive vascular contractility. For that purpose, contractile activity of cultured bovine aortic smooth muscle cells (BASMCs) embedded in collagen gels was monitored by changes in gel diameter. After culturing for 5 days, ATP- and high KCl solution-induced contractions were significantly enhanced in the gels that were overlaid with bovine aortic endothelial cells (BAECs) or were cultured with conditioned medium of cultured BAECs. ATP-induced Ca2+ transients, recorded in BASMCs cultured with conditioned medium of BAECs, were markedly augmented, but high KCl-induced Ca2+ transients were not affected. BASMCs in control gels were spindle shaped, and those in endothelium-treated gels were more elongated and interconnected. The endothelial conditioned medium also strongly affected the intracellular distribution of actin fibers. Conditioned medium of BAECs contained TGFβ1 and TGFβ2. The TGFβ receptor antagonist SB431542 as well as simultaneous treatment with TGFβ1 and TGFβ2 neutralizing antibodies completely reversed the above effects of endothelial conditioned medium on BASMCs. BAECs medium induced phosphorylation of Smad2 and increased ATP-induced phosphorylation of myosin light chain in BASMCs. The present results indicate that the release of TGFβ1 and TGFβ2 from vascular endothelium affects the contractility of vascular smooth muscle cells by altering their morphology and agonist-induced Ca2+ mobilization.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalPflugers Archiv European Journal of Physiology
Volume466
Issue number2
DOIs
Publication statusPublished - Feb 1 2014

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Transforming Growth Factors
Vascular Smooth Muscle
Endothelium
Muscle
Endothelial cells
Conditioned Culture Medium
Smooth Muscle Myocytes
Gels
Proteins
Endothelial Cells
Phosphorylation
Adenosine Triphosphate
Vascular Endothelium
Cells
Myosin Light Chains
Vasoconstrictor Agents
Neutralizing Antibodies
Vasodilator Agents
Actins
Collagen

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Development of vascular smooth muscle contractility by endothelium-derived transforming growth factor β proteins. / Kimura, Chiwaka; Konishi, Shuhei; Hasegawa, Maki; Oike, Masahiro.

In: Pflugers Archiv European Journal of Physiology, Vol. 466, No. 2, 01.02.2014, p. 369-380.

Research output: Contribution to journalArticle

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