Abstract
In the present study, we compared the expression of cyclin D2 and D2SV, the cyclin D2 splice variant, in mouse cerebellum at postnatal day 1 (P1), P7, P14 and P28. Western blotting revealed that cyclin D2 levels (34. kDa) peaked at P7 and then decreased to levels near the limit of detection at P28. To detect D2SV, we generated a rabbit polyclonal antibody directed against a C-terminal motif unique to this protein that recognizes two D2SV immunoreactive bands at 20 and 25. kDa. Western blotting indicated that levels of the 20. kDa band remained constant from P1 to P28 while the intensity of the 25. kDa band decreased gradually over this period of time. At P7, cyclin D2 immunoreactivity was evident throughout the cerebellum where it was located in nestin-positive cells. By contrast, at P28, cyclin D2 immunoreactivity was restricted to Bergmann glia whose cell bodies are located in the Purkinje cell layer and processes extend into the molecular layer. Unlike cyclin D2, D2SV immunoreactivity was restricted to Purkinje cells at both P7 and P28. Our observations that D2SV immunoreactivity is localized to Purkinje cells and reflects changing levels of at least two D2SV immunoreactive proteins suggests that this splice variant may play an important role in the postnatal development of these neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 100-104 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 477 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 1 2010 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Cite this
Developmental expression of the cyclin D2 splice variant in postnatal Purkinje cells of the mouse cerebellum. / Kajitani, Kosuke; Wafa, Karim; Pasumarthi, Kishore B.S.; Robertson, George S.
In: Neuroscience Letters, Vol. 477, No. 2, 01.06.2010, p. 100-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Developmental expression of the cyclin D2 splice variant in postnatal Purkinje cells of the mouse cerebellum
AU - Kajitani, Kosuke
AU - Wafa, Karim
AU - Pasumarthi, Kishore B.S.
AU - Robertson, George S.
PY - 2010/6/1
Y1 - 2010/6/1
N2 - In the present study, we compared the expression of cyclin D2 and D2SV, the cyclin D2 splice variant, in mouse cerebellum at postnatal day 1 (P1), P7, P14 and P28. Western blotting revealed that cyclin D2 levels (34. kDa) peaked at P7 and then decreased to levels near the limit of detection at P28. To detect D2SV, we generated a rabbit polyclonal antibody directed against a C-terminal motif unique to this protein that recognizes two D2SV immunoreactive bands at 20 and 25. kDa. Western blotting indicated that levels of the 20. kDa band remained constant from P1 to P28 while the intensity of the 25. kDa band decreased gradually over this period of time. At P7, cyclin D2 immunoreactivity was evident throughout the cerebellum where it was located in nestin-positive cells. By contrast, at P28, cyclin D2 immunoreactivity was restricted to Bergmann glia whose cell bodies are located in the Purkinje cell layer and processes extend into the molecular layer. Unlike cyclin D2, D2SV immunoreactivity was restricted to Purkinje cells at both P7 and P28. Our observations that D2SV immunoreactivity is localized to Purkinje cells and reflects changing levels of at least two D2SV immunoreactive proteins suggests that this splice variant may play an important role in the postnatal development of these neurons.
AB - In the present study, we compared the expression of cyclin D2 and D2SV, the cyclin D2 splice variant, in mouse cerebellum at postnatal day 1 (P1), P7, P14 and P28. Western blotting revealed that cyclin D2 levels (34. kDa) peaked at P7 and then decreased to levels near the limit of detection at P28. To detect D2SV, we generated a rabbit polyclonal antibody directed against a C-terminal motif unique to this protein that recognizes two D2SV immunoreactive bands at 20 and 25. kDa. Western blotting indicated that levels of the 20. kDa band remained constant from P1 to P28 while the intensity of the 25. kDa band decreased gradually over this period of time. At P7, cyclin D2 immunoreactivity was evident throughout the cerebellum where it was located in nestin-positive cells. By contrast, at P28, cyclin D2 immunoreactivity was restricted to Bergmann glia whose cell bodies are located in the Purkinje cell layer and processes extend into the molecular layer. Unlike cyclin D2, D2SV immunoreactivity was restricted to Purkinje cells at both P7 and P28. Our observations that D2SV immunoreactivity is localized to Purkinje cells and reflects changing levels of at least two D2SV immunoreactive proteins suggests that this splice variant may play an important role in the postnatal development of these neurons.
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U2 - 10.1016/j.neulet.2010.04.042
DO - 10.1016/j.neulet.2010.04.042
M3 - Article
VL - 477
SP - 100
EP - 104
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -