Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturases in wheat (Triticum aestivum L.)

Gorou Horiguchi, Naoto Kawakami, Kensuke Kusumi, Hiroaki Kodama, Koh Iba

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

ω-3 fatty acid desaturase is responsible for the production of α-linolenic acid, which is a major fatty acid constituent of membrane lipids in higher plants. In this study, the developmental regulation of the expression of two genes, TaFAD3 and TaFAD7, which respectively encode the microsome and plastid ω-3 fatty acid desaturases in wheat, was analyzed by in situ hybridization. The transcript of TaFAD3 was abundant in the apical meristems of both shoot and root, the leaf primordium, the leaf basal intercalary meristem, and the root vascular tissues. The level of TaFAD3 mRNA was also high in embryos developing after flowering. In mature embryos, however, it was low, whereas in germinating embryos it was once again high. These results suggest that the TaFAD3 mRNA is accumulated at a high level in cells that have an ability to proliferate. In contrast, the TaFAD7 mRNA level was very low in the shoot apical meristem, leaf primordium and leaf basal intercalary meristem, but a large accumulation of TaFAD7 mRNA was found in green tissues, suggesting that the accumulation of this mRNA is associated with chloroplast development. Although the spatial distribution patterns of TaFAD3 and TaFAD7 mRNAs clearly differed, the enhanced accumulation of the TaFAD3 and TaFAD7 mRNAs was linked with the active membrane biogenesis required for cell division and with the development of the thylakoid system in plastids, respectively.

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalPlant and Cell Physiology
Volume39
Issue number5
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Fatty Acid Desaturases
Developmental Genes
stearoyl-CoA desaturase
Plastids
microsomes
Microsomes
embryo (plant)
Triticum
plastids
leaf primordia
Triticum aestivum
apical meristems
Meristem
meristems
Messenger RNA
wheat
genes
shoot meristems
vascular tissues
linolenic acid

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturases in wheat (Triticum aestivum L.). / Horiguchi, Gorou; Kawakami, Naoto; Kusumi, Kensuke; Kodama, Hiroaki; Iba, Koh.

In: Plant and Cell Physiology, Vol. 39, No. 5, 01.01.1998, p. 540-544.

Research output: Contribution to journalArticle

@article{0ce177ef89744029beaf0a77b72f0bc2,
title = "Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturases in wheat (Triticum aestivum L.)",
abstract = "ω-3 fatty acid desaturase is responsible for the production of α-linolenic acid, which is a major fatty acid constituent of membrane lipids in higher plants. In this study, the developmental regulation of the expression of two genes, TaFAD3 and TaFAD7, which respectively encode the microsome and plastid ω-3 fatty acid desaturases in wheat, was analyzed by in situ hybridization. The transcript of TaFAD3 was abundant in the apical meristems of both shoot and root, the leaf primordium, the leaf basal intercalary meristem, and the root vascular tissues. The level of TaFAD3 mRNA was also high in embryos developing after flowering. In mature embryos, however, it was low, whereas in germinating embryos it was once again high. These results suggest that the TaFAD3 mRNA is accumulated at a high level in cells that have an ability to proliferate. In contrast, the TaFAD7 mRNA level was very low in the shoot apical meristem, leaf primordium and leaf basal intercalary meristem, but a large accumulation of TaFAD7 mRNA was found in green tissues, suggesting that the accumulation of this mRNA is associated with chloroplast development. Although the spatial distribution patterns of TaFAD3 and TaFAD7 mRNAs clearly differed, the enhanced accumulation of the TaFAD3 and TaFAD7 mRNAs was linked with the active membrane biogenesis required for cell division and with the development of the thylakoid system in plastids, respectively.",
author = "Gorou Horiguchi and Naoto Kawakami and Kensuke Kusumi and Hiroaki Kodama and Koh Iba",
year = "1998",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.pcp.a029402",
language = "English",
volume = "39",
pages = "540--544",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturases in wheat (Triticum aestivum L.)

AU - Horiguchi, Gorou

AU - Kawakami, Naoto

AU - Kusumi, Kensuke

AU - Kodama, Hiroaki

AU - Iba, Koh

PY - 1998/1/1

Y1 - 1998/1/1

N2 - ω-3 fatty acid desaturase is responsible for the production of α-linolenic acid, which is a major fatty acid constituent of membrane lipids in higher plants. In this study, the developmental regulation of the expression of two genes, TaFAD3 and TaFAD7, which respectively encode the microsome and plastid ω-3 fatty acid desaturases in wheat, was analyzed by in situ hybridization. The transcript of TaFAD3 was abundant in the apical meristems of both shoot and root, the leaf primordium, the leaf basal intercalary meristem, and the root vascular tissues. The level of TaFAD3 mRNA was also high in embryos developing after flowering. In mature embryos, however, it was low, whereas in germinating embryos it was once again high. These results suggest that the TaFAD3 mRNA is accumulated at a high level in cells that have an ability to proliferate. In contrast, the TaFAD7 mRNA level was very low in the shoot apical meristem, leaf primordium and leaf basal intercalary meristem, but a large accumulation of TaFAD7 mRNA was found in green tissues, suggesting that the accumulation of this mRNA is associated with chloroplast development. Although the spatial distribution patterns of TaFAD3 and TaFAD7 mRNAs clearly differed, the enhanced accumulation of the TaFAD3 and TaFAD7 mRNAs was linked with the active membrane biogenesis required for cell division and with the development of the thylakoid system in plastids, respectively.

AB - ω-3 fatty acid desaturase is responsible for the production of α-linolenic acid, which is a major fatty acid constituent of membrane lipids in higher plants. In this study, the developmental regulation of the expression of two genes, TaFAD3 and TaFAD7, which respectively encode the microsome and plastid ω-3 fatty acid desaturases in wheat, was analyzed by in situ hybridization. The transcript of TaFAD3 was abundant in the apical meristems of both shoot and root, the leaf primordium, the leaf basal intercalary meristem, and the root vascular tissues. The level of TaFAD3 mRNA was also high in embryos developing after flowering. In mature embryos, however, it was low, whereas in germinating embryos it was once again high. These results suggest that the TaFAD3 mRNA is accumulated at a high level in cells that have an ability to proliferate. In contrast, the TaFAD7 mRNA level was very low in the shoot apical meristem, leaf primordium and leaf basal intercalary meristem, but a large accumulation of TaFAD7 mRNA was found in green tissues, suggesting that the accumulation of this mRNA is associated with chloroplast development. Although the spatial distribution patterns of TaFAD3 and TaFAD7 mRNAs clearly differed, the enhanced accumulation of the TaFAD3 and TaFAD7 mRNAs was linked with the active membrane biogenesis required for cell division and with the development of the thylakoid system in plastids, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0031780286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031780286&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.pcp.a029402

DO - 10.1093/oxfordjournals.pcp.a029402

M3 - Article

AN - SCOPUS:0031780286

VL - 39

SP - 540

EP - 544

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 5

ER -