Developmental regulation of ubiquitin C-terminal hydrolase isozyme expression during spermatogenesis in mice

Jungkee Kwon, Yu Lai Wang, Rieko Setsuie, Satoshi Sekiguchi, Mikako Sakurai, Yae Sato, Won Woo Lee, Yoshiyuki Ishii, Shigeru Kyuwa, Mami Noda, Keiji Wada, Yasuhiro Yoshikawa

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The ubiquitin pathway functions in the process of protein turnover in eukaryotic cells. This pathway comprises the enzymes that ubiquitinate/ deubiquitinate target proteins and the proteasome that degrades ubiquitin-conjugated proteins. Ubiquitin C-terminal hydrolases (UCHs) are thought to be essential for maintaining ubiquitination activity by releasing ubiquitin (Ub) from its substrates. Mammalian UCH-L1 and UCH-L3 are small proteins that share considerable homology at the amino acid level. Both of these UCHs are highly expressed in the testis/ ovary and neuronal cells. Our previous work demonstrated that UCH-L1-deficient gracile axonal dystrophy (gad) mice exhibit progressively decreasing spermatogonial stem cell proliferation, suggesting that UCH isozymes in the testis function during spermatogenesis. To analyze the expression patterns of UCH isozymes during spermatogenesis, we isolated nearly homogeneous populations of spermatogonia, spermatocytes, spermatids, and Sertoli cells from mouse testes. Western blot analysis detected UCH-L1 in spermatogonia and Sertoli cells, whereas UCH-L3 was detected in spermatocytes and spermatids. Moreover, reverse transcription-polymerase chain reaction analysis of UCH isozymes showed that UCH-L1 and UCH-L4 mRNAs are expressed in spermatogonia, whereas UCH-L3 and UCH-L5 mRNAs are expressed mainly in spermatocytes and spermatids. These results suggest that UCH-L1 and UCH-L3 have distinct functions during spermatogenesis, namely, that UCH-L1 may act during mitotic proliferation of spermatogonial stem cells whereas UCH-L3 may function in the meiotic differentiation of spermatocytes into spermatids.

Original languageEnglish
Pages (from-to)515-521
Number of pages7
JournalBiology of Reproduction
Volume71
Issue number2
DOIs
Publication statusPublished - Aug 1 2004

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Ubiquitin Thiolesterase
Spermatogenesis
Isoenzymes
Spermatocytes
Spermatids
Spermatogonia
Ubiquitin
Testis
Sertoli Cells
Proteins
Stem Cells

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Cite this

Kwon, J., Wang, Y. L., Setsuie, R., Sekiguchi, S., Sakurai, M., Sato, Y., ... Yoshikawa, Y. (2004). Developmental regulation of ubiquitin C-terminal hydrolase isozyme expression during spermatogenesis in mice. Biology of Reproduction, 71(2), 515-521. https://doi.org/10.1095/biolreprod.104.027565

Developmental regulation of ubiquitin C-terminal hydrolase isozyme expression during spermatogenesis in mice. / Kwon, Jungkee; Wang, Yu Lai; Setsuie, Rieko; Sekiguchi, Satoshi; Sakurai, Mikako; Sato, Yae; Lee, Won Woo; Ishii, Yoshiyuki; Kyuwa, Shigeru; Noda, Mami; Wada, Keiji; Yoshikawa, Yasuhiro.

In: Biology of Reproduction, Vol. 71, No. 2, 01.08.2004, p. 515-521.

Research output: Contribution to journalArticle

Kwon, J, Wang, YL, Setsuie, R, Sekiguchi, S, Sakurai, M, Sato, Y, Lee, WW, Ishii, Y, Kyuwa, S, Noda, M, Wada, K & Yoshikawa, Y 2004, 'Developmental regulation of ubiquitin C-terminal hydrolase isozyme expression during spermatogenesis in mice', Biology of Reproduction, vol. 71, no. 2, pp. 515-521. https://doi.org/10.1095/biolreprod.104.027565
Kwon, Jungkee ; Wang, Yu Lai ; Setsuie, Rieko ; Sekiguchi, Satoshi ; Sakurai, Mikako ; Sato, Yae ; Lee, Won Woo ; Ishii, Yoshiyuki ; Kyuwa, Shigeru ; Noda, Mami ; Wada, Keiji ; Yoshikawa, Yasuhiro. / Developmental regulation of ubiquitin C-terminal hydrolase isozyme expression during spermatogenesis in mice. In: Biology of Reproduction. 2004 ; Vol. 71, No. 2. pp. 515-521.
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