Developmental switch from GABA to glycine release in single central synaptic terminals

Junichi Nabekura, Shutaro Katsurabayashi, Yasuhiro Kakazu, Shumei Shibata, Atsushi Matsubara, Shozo Jinno, Yoshito Mizoguchi, Akira Sasaki, Hitoshi Ishibashi

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Early in postnatal development, inhibitory inputs to rat lateral superior olive (LSO) neurons change from releasing predominantly GABA to releasing predominantly glycine into the synapse. Here we show that spontaneous miniature inhibitory postsynaptic currents (mIPSCs) also change from GABAergic to glycinergic over the first two postnatal weeks. Many 'mixed' mIPSCs, resulting from corelease of glycine and GABA from the same vesicles, are seen during this transition. Immunohistochemistry showed that a large number of terminals contained both GABA and glycine at postnatal day 8 (P8). By P14, both the content of GABA in these mixed terminals and the contribution of GABA to the mixed mIPSCs had decreased. The content of glycine in terminals increased over the same period. Our results indicate that switching from GABAergic to glycinergic inputs to the LSO may occur at the level of a single presynaptic terminal. This demonstrates a new form of developmental plasticity at the level of a single central synapse.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalNature Neuroscience
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

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Presynaptic Terminals
Glycine
gamma-Aminobutyric Acid
Inhibitory Postsynaptic Potentials
Synapses
Immunohistochemistry
Neurons
Superior Olivary Complex

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Nabekura, J., Katsurabayashi, S., Kakazu, Y., Shibata, S., Matsubara, A., Jinno, S., ... Ishibashi, H. (2004). Developmental switch from GABA to glycine release in single central synaptic terminals. Nature Neuroscience, 7(1), 17-23. https://doi.org/10.1038/nn1170

Developmental switch from GABA to glycine release in single central synaptic terminals. / Nabekura, Junichi; Katsurabayashi, Shutaro; Kakazu, Yasuhiro; Shibata, Shumei; Matsubara, Atsushi; Jinno, Shozo; Mizoguchi, Yoshito; Sasaki, Akira; Ishibashi, Hitoshi.

In: Nature Neuroscience, Vol. 7, No. 1, 01.01.2004, p. 17-23.

Research output: Contribution to journalArticle

Nabekura, J, Katsurabayashi, S, Kakazu, Y, Shibata, S, Matsubara, A, Jinno, S, Mizoguchi, Y, Sasaki, A & Ishibashi, H 2004, 'Developmental switch from GABA to glycine release in single central synaptic terminals', Nature Neuroscience, vol. 7, no. 1, pp. 17-23. https://doi.org/10.1038/nn1170
Nabekura J, Katsurabayashi S, Kakazu Y, Shibata S, Matsubara A, Jinno S et al. Developmental switch from GABA to glycine release in single central synaptic terminals. Nature Neuroscience. 2004 Jan 1;7(1):17-23. https://doi.org/10.1038/nn1170
Nabekura, Junichi ; Katsurabayashi, Shutaro ; Kakazu, Yasuhiro ; Shibata, Shumei ; Matsubara, Atsushi ; Jinno, Shozo ; Mizoguchi, Yoshito ; Sasaki, Akira ; Ishibashi, Hitoshi. / Developmental switch from GABA to glycine release in single central synaptic terminals. In: Nature Neuroscience. 2004 ; Vol. 7, No. 1. pp. 17-23.
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