Developmentally-regulated expression of mNapor encoding an apoptosis-induced ELAV-type RNA binding protein

Dong Kug Choi, Takashi Ito, Fujiko Tsukahara, Momoki Hirai, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

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Abstract

Proteins with RNA recognition motifs (RRMs) participate in many aspects of RNA metabolism, and some of them are required for the accomplishment of normal development. The neuroblastoma apoptosis-related RNA binding protein (NAPOR) is an ELAV-type RNA-binding protein with three characteristic RNP2/RNP1-type RRMs, which we identified as a gene induced during apoptosis of neuroblastoma cells. Here we isolated and characterized the cDNA for mNapor, the mouse homolog of NAPOR. The mNapor encodes mRNA sharing striking homology with that of NAPOR, not only in its open reading frame (98.5%) but also in the 3'-untranslated region (80.1%), and is mapped to chromosome 2 A2-A3, a region syntenic to the human NAPOR locus. In situ hybridization analysis revealed that the expression pattern of mNapor is spatially and temporally coincident with the occurrence of programmed cell death, suggesting its involvement in the development of the central nervous system in which apoptosis plays a crucial role. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalGene
Volume237
Issue number1
DOIs
Publication statusPublished - Sep 3 1999

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RNA-Binding Proteins
Neuroblastoma
Apoptosis
Chromosomes, Human, Pair 2
3' Untranslated Regions
varespladib methyl
Open Reading Frames
In Situ Hybridization
Cell Death
Central Nervous System
Complementary DNA
RNA
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Developmentally-regulated expression of mNapor encoding an apoptosis-induced ELAV-type RNA binding protein. / Choi, Dong Kug; Ito, Takashi; Tsukahara, Fujiko; Hirai, Momoki; Sakaki, Yoshiyuki.

In: Gene, Vol. 237, No. 1, 03.09.1999, p. 135-142.

Research output: Contribution to journalArticle

Choi, Dong Kug ; Ito, Takashi ; Tsukahara, Fujiko ; Hirai, Momoki ; Sakaki, Yoshiyuki. / Developmentally-regulated expression of mNapor encoding an apoptosis-induced ELAV-type RNA binding protein. In: Gene. 1999 ; Vol. 237, No. 1. pp. 135-142.
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