Developmentally regulated O-acetylated sialoglycans in the central nervous system revealed by a new monoclonal antibody 493D4 recognizing a wide range of O-acetylated glycoconjugates

Gu Zhang, Li Ji, Sadamu Kurono, Shinobu C. Fujita, Shigeki Furuya, Yoshio Hirabayashi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have previously detected an alkali-labile and developmentally regulated antigen in rat embryonic cerebral cortex, which may be 9-O-acetylsialylated GT3 ganglioside (Hirabayashi Y, Hirota M, Suzuki Y, Matsumoto M, Obata K, Ando S (1989) Neurosci Lett 106:193-98). In this study we established a mouse monoclonal antibody, 493D4, that recognizes 9-O-acetyl GT3 ganglioside, but not non-O-acetyl gangliosides. This antibody also reacted with 9-O acetyl GD3 to a much lesser extent. By using this antibody, we found that O-acetyl GT3 as well as O-acetyl GD3 were expressed strongly in fetal murine cerebral cortex and decreased to an undetectable level after birth. With the assistance of TLC-immunostaining using 493D4 together with Q-Sepharose column chromatography, O-acetyl gangliosides of bovine brain were purified and the structural analysis showed the presence of O-acetyl GD3, O-acetyl LD1, O-acetyl GD2 and O-acetyl GD1b in the adult brain as extremely minor components. Interestingly, the antibody 493D4 could detect O-acetyl sialoglycoproteins in rat brain tissues. One of the major immunoreactive proteins was shown to be synaptophysin, an integral membrane protein specifically present in synaptic vesicles. This monoclonal antibody was therefore useful for sensitive detection of both O-acetylated gangliosides and glycoproteins with O-acetylated sialic acids.

Original languageEnglish
Pages (from-to)847-857
Number of pages11
JournalGlycoconjugate Journal
Volume14
Issue number7
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

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Glycoconjugates
Gangliosides
Neurology
Brain
Central Nervous System
Monoclonal Antibodies
Cerebral Cortex
Antibodies
Rats
Sialoglycoproteins
Sialic Acids
Agarose Chromatography
Synaptophysin
Column chromatography
Synaptic Vesicles
Alkalies
Structural analysis
Sepharose
Glycoproteins
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Developmentally regulated O-acetylated sialoglycans in the central nervous system revealed by a new monoclonal antibody 493D4 recognizing a wide range of O-acetylated glycoconjugates. / Zhang, Gu; Ji, Li; Kurono, Sadamu; Fujita, Shinobu C.; Furuya, Shigeki; Hirabayashi, Yoshio.

In: Glycoconjugate Journal, Vol. 14, No. 7, 01.01.1997, p. 847-857.

Research output: Contribution to journalArticle

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